{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T21:46:04Z","timestamp":1784324764275,"version":"3.55.0"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2014,3,12]],"date-time":"2014-03-12T00:00:00Z","timestamp":1394582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100006092","name":"Clinical Laboratory, Rosalind Franklin University of Medicine and Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006092","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,3,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>a2V is required for maturation of sperm. The decreased expression of a2V at the feto-maternal interphase causes poor pregnancy outcome. The present study examined the role of a2V in spermatogenesis and inflammatory network in the testis. A single dose of anti-a2V mouse IgG or mouse IgG isotype (3 \u03bcg\/animal) was injected i.p. into male mice on alternate days for 10 days. Anti-a2V-treated males exhibit severe deficiencies of spermatogenesis, which is indicated by the presence of less numbers of postmeiotic cells. Sperm counts and sperm motility were reduced significantly in anti-a2V-treated males. The release of the cleaved a2NTD was significantly lower in anti-a2V-treated testes. The TMs were identified as M2-like macrophages, and this population and the expression of various cytokines\/chemokines (Tgf-\u03b2, Il-6, Nos2, Tnf, Lif, Mcp1, Ccl5) were decreased significantly in anti-a2V-treated testis compared with control testis. Moreover, the cleaved a2NTD acts as a key mediator of TMs and significantly up-regulates the secretion of testicular cytokines\/chemokines, which are associated with normal spermatogenesis. When these anti-a2V-treated males were used for mating with normal females, the number of implantation sites was decreased significantly in the females mated with anti-a2V-treated males than the females mated with control males. These observations suggest that a2V plays a crucial role in spermatogenesis by regulating testicular immune responses, and its inhibition in males leads to poor pregnancy outcome in females.<\/jats:p>","DOI":"10.1189\/jlb.3a1113-593rr","type":"journal-article","created":{"date-parts":[[2014,3,13]],"date-time":"2014-03-13T02:34:42Z","timestamp":1394678082000},"page":"337-347","source":"Crossref","is-referenced-by-count":19,"title":["Vacuolar-ATPase isoform a2 regulates macrophages and cytokine profile necessary for normal spermatogenesis in testis"],"prefix":"10.1093","volume":"96","author":[{"given":"Mukesh K","family":"Jaiswal","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Rosalind Franklin University of Medicine and Science , North Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gajendra K","family":"Katara","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Rosalind Franklin University of Medicine and Science , North Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy","family":"Mallers","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Rosalind Franklin University of Medicine and Science , North Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard","family":"Chaouat","sequence":"additional","affiliation":[{"name":"U976 Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale\/Centre National de la Recherche Scientifique, St. Louis Hospital , Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice","family":"Gilman-Sachs","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Rosalind Franklin University of Medicine and Science , North Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth D","family":"Beaman","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Rosalind Franklin University of Medicine and Science , North Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2014,3,12]]},"reference":[{"key":"2023032708483990300_","doi-asserted-by":"crossref","first-page":"713","DOI":"10.1530\/REP-12-0036","article-title":"V-ATPase upregulation during early pregnancy: a possible link to establishment of an inflammatory response during preimplantation period of pregnancy","volume":"143","author":"Jaiswal","year":"2012","journal-title":"Reproduction"},{"key":"2023032708483990300_","doi-asserted-by":"crossref","first-page":"626","DOI":"10.1095\/biolreprod.111.092494","article-title":"Placental ATPase expression is a link between multiple causes of spontaneous abortion in mice","volume":"85","author":"Jaiswal","year":"2011","journal-title":"Biol. 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