{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T04:17:27Z","timestamp":1791001047437,"version":"4.1.0"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2016,2,11]],"date-time":"2016-02-11T00:00:00Z","timestamp":1455148800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"R.B."},{"name":"L.L."},{"DOI":"10.13039\/100000002","name":"U.S. National Institutes of Health","doi-asserted-by":"publisher","award":["NIH-AR049610"],"award-info":[{"award-number":["NIH-AR049610"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This study evaluated the role of macrophage migration inhibitory factor in inflammation caused by monosodium urate crystals. The concentration of macrophage migration inhibitory factor was increased in synovial fluid of patients with acute gout, and there was a positive correlation between intra-articular macrophage migration inhibitory factor and IL-1\u03b2 concentrations. In mice, the injection of monosodium urate crystals into the knee joint increased the levels of macrophage migration inhibitory factor in macrophages and in inflamed tissue. The injection of recombinant macrophage migration inhibitory factor into the joint of mice reproduced the inflammatory response observed in acute gout, including histologic changes, the recruitment of neutrophils, and increased levels of IL-1\u03b2 and CXCL1. Importantly, the accumulation of neutrophils and the amount IL-1\u03b2 in the joints were reduced in macrophage migration inhibitory factor-deficient mice when injected with monosodium urate crystals. We observed a similar effect when we blocked macrophage migration inhibitory factor with (S,R)-3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazole acetic acid or anti-macrophage migration inhibitory factor. In addition, the blockade of IL-1R and CXCR2 reduced recombinant macrophage migration inhibitory factor-induced neutrophil recruitment. Mechanistically, recombinant macrophage migration inhibitory factor is important for the synthesis of il1\u03b2 mRNA in vivo and in isolated macrophages. Altogether, macrophage migration inhibitory factor promotes neutrophil accumulation and is important for IL-1\u03b2 production, which are 2 crucial events contributing to the pathogenesis of acute gout.<\/jats:p>","DOI":"10.1189\/jlb.3ma0915-418r","type":"journal-article","created":{"date-parts":[[2016,2,12]],"date-time":"2016-02-12T03:24:22Z","timestamp":1455247462000},"page":"1035-1043","source":"Crossref","is-referenced-by-count":47,"title":["Macrophage migration inhibitory factor drives neutrophil accumulation by facilitating IL-1\u03b2 production in a murine model of acute gout"],"prefix":"10.1093","volume":"99","author":[{"given":"Izabela","family":"Galv\u00e3o","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Immunology, Institute of Biological Sciences, Universidade Federal de Minas Gerais , Belo Horizonte, Minas Gerais , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Carolina 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Institute of Biological Sciences, Universidade Federal de Minas Gerais , Belo Horizonte, Minas Gerais , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivian Vasconcelos","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais , Belo Horizonte, Minas Gerais , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alesandra Corte","family":"Reis","sequence":"additional","affiliation":[{"name":"Department of Morphology, Institute of Biological Sciences, Universidade Federal de Minas Gerais , Belo Horizonte, Minas Gerais , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene Donizeti","family":"Ribeiro Oliveira","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, School of Medicine of Ribeirao Preto, Universidade de S\u00e3o Paulo , 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