{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:23:06Z","timestamp":1790518986496,"version":"4.1.0"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2016,2,16]],"date-time":"2016-02-16T00:00:00Z","timestamp":1455580800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Fundac\u00e3o de Apoio \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2008\/05982-8"],"award-info":[{"award-number":["2008\/05982-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Fundac\u00e3o de Apoio \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2007\/53940-0"],"award-info":[{"award-number":["2007\/53940-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Institute of Science and Technology in Vaccines"},{"DOI":"10.13039\/501100003593","name":"National Council of Scientific and Technological Development","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In vertebrate hosts, Leishmania braziliensis parasites infect mainly mononuclear phagocytic system cells, which when activated by T helper cell type 1 cytokines produce nitric oxide and kill the pathogens. Chemokine (C\u2013C motif) receptor 2 is a chemokine receptor that binds primarily chemokine (C\u2013C motif) ligand 2 and has an important role in the recruitment of monocytic phagocytes. Although it has been reported that Leishmania braziliensis infection induces CCR2 expression in the lesions, the role of CCR2 during Leishmania braziliensis infection remains unknown. Here, we showed that CCR2 has a role in mediating protection against Leishmania braziliensis infection in mice. The absence of CCR2 resulted in increased susceptibility to infection and was associated with low amounts of Ly6C+ inflammatory dendritic cells in the lesions, which we found to be the major sources of tumor necrosis factor production and induced nitric oxide synthase expression in C57BL\/6 mice lesions. Consequently, CCR2\u2212\/\u2212 mice showed decreased tumor necrosis factor production and induced nitric oxide synthase expression, resulting in impaired parasite elimination. We also demonstrated that CCR2 has a role in directly mediating the differentiation of monocytes into inflammatory dendritic cells at the infection sites, contributing to the accumulation of inflammatory dendritic cells in Leishmania braziliensis lesions and subsequent control of parasite replication. Therefore, these data provide new information on the role of chemokines during the immune response to infections and identify a potential target for therapeutic interventions in cutaneous leishmaniasis.<\/jats:p>","DOI":"10.1189\/jlb.4a0715-288r","type":"journal-article","created":{"date-parts":[[2016,2,17]],"date-time":"2016-02-17T03:58:33Z","timestamp":1455681513000},"page":"423-432","source":"Crossref","is-referenced-by-count":16,"title":["CCR2 signaling contributes to the differentiation of protective inflammatory dendritic cells in <i>Leishmania braziliensis<\/i> infection"],"prefix":"10.1093","volume":"100","author":[{"given":"Diego L","family":"Costa","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Immunology, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo , Ribeir\u00e3o Preto, SP , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Djalma S","family":"Lima-J\u00fanior","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo , Ribeir\u00e3o Preto, SP , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela S","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo , Ribeir\u00e3o Preto, SP , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"La\u00eds A","family":"Sacramento","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo , Ribeir\u00e3o Preto, SP , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roque P","family":"Almeida","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Pathology, Center for Biological and Health Sciences, Federal University of Sergipe , Aracaj\u00fa, SE , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Carregaro","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo , Ribeir\u00e3o Preto, SP , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o S","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo , Ribeir\u00e3o Preto, SP , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2016,2,16]]},"reference":[{"key":"2023011710413523600_B1","doi-asserted-by":"crossref","first-page":"2993","DOI":"10.1242\/jcs.112.18.2993","article-title":"Leishmania species: models of intracellular parasitism","volume":"112","author":"Alexander","year":"1999","journal-title":"J. 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