{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T00:05:50Z","timestamp":1785197150989,"version":"3.55.0"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T00:00:00Z","timestamp":1675036800000},"content-version":"vor","delay-in-days":2839,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009950","name":"Ministry of Education","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009950","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007951","name":"Tokyo Medical and Dental University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007951","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shiori Tanimura for technical assistance"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Autophagy occurs in human neutrophils after the phagocytosis of multidrug-resistant bacteria and drug-sensitive strains, including Escherichia coli and Pseudomonas aeruginosa. The present study detected autophagy by immunoblot analysis of LC3B conversion, by confocal scanning microscopic examination of LC3B aggregate formation and by transmission electron microscopic examination of bacteria-containing autophagosomes. Patients with severe bacterial infections are often treated with IVIG alongside antimicrobial agents. Here, we showed that IVIG induced neutrophil-mediated phagocytosis of multidrug-resistant strains. Compared with untreated neutrophils, neutrophils exposed to IVIG showed increased levels of bacterial cell killing, phagocytosis, O2\u2212 release, MPO release, and NET formation. IVIG also increased autophagy in these cells. Inhibiting the late phase of autophagy (fusion of lysosomes with autophagosomes) with bafilomycin A1-reduced, neutrophil-mediated bactericidal activity. These findings indicate that autophagy plays a critical role in the bactericidal activity mediated by human neutrophils. Furthermore, the autophagosomes within the neutrophils contained bacteria only and their organelles only, or both bacteria and their organelles, a previously undocumented observation. Taken together, these results suggest that the contents of neutrophil autophagosomes may be derived from specific autophagic systems, which provide the neutrophil with an advantage. Thus, IVIG promotes the neutrophil-mediated killing of multidrug-resistant bacteria as well as drug-sensitive strains.<\/jats:p>","DOI":"10.1189\/jlb.4a0813-422rrr","type":"journal-article","created":{"date-parts":[[2015,4,24]],"date-time":"2015-04-24T03:21:14Z","timestamp":1429845674000},"page":"107-117","source":"Crossref","is-referenced-by-count":31,"title":["Enhancement of neutrophil autophagy by an IVIG preparation against multidrug-resistant bacteria as well as drug-sensitive strains"],"prefix":"10.1093","volume":"98","author":[{"given":"Hiroshi","family":"Itoh","sequence":"first","affiliation":[{"name":"Department of Human Health Sciences, Graduate School of Medicine, Kyoto University , Kyoto , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidemasa","family":"Matsuo","sequence":"additional","affiliation":[{"name":"Department of Human Health Sciences, Graduate School of Medicine, Kyoto University , Kyoto , Japan"},{"name":"Department of Clinical Laboratory, Kyoto University Hospital , Kyoto , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoko","family":"Kitamura","sequence":"additional","affiliation":[{"name":"Department of Human Health Sciences, Graduate School of Medicine, Kyoto University , Kyoto , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sho","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Human Health Sciences, Graduate School of Medicine, Kyoto University , Kyoto , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Higuchi","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Kyoto University Hospital , Kyoto , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromu","family":"Takematsu","sequence":"additional","affiliation":[{"name":"Department of Human Health Sciences, Graduate School of Medicine, Kyoto University , Kyoto , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiko","family":"Kamikubo","sequence":"additional","affiliation":[{"name":"Department of Human Health Sciences, Graduate School of Medicine, Kyoto University , Kyoto , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadakazu","family":"Kondo","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University , Kyoto , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kouhei","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University , Kyoto , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masataka","family":"Sasada","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology, Shiga Medical Center for Adults , Shiga , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akifumi","family":"Takaori-Kondo","sequence":"additional","affiliation":[{"name":"Department of Hematology and Oncology, Graduate School of Medicine, Kyoto University , Kyoto , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Souichi","family":"Adachi","sequence":"additional","affiliation":[{"name":"Department of Human Health Sciences, Graduate School of Medicine, Kyoto University , Kyoto , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2015,4,23]]},"reference":[{"key":"2023013012472675500_B1","first-page":"199","article-title":"The efficacy of intravenous immunoglobulin in combination therapy with antibiotics for severe infections","volume":"48","author":"Masaoka","year":"2000","journal-title":"Jpn. 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