{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T20:35:48Z","timestamp":1761597348000},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2012,7,1]],"date-time":"2012-07-01T00:00:00Z","timestamp":1341100800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Fondo de Investigaciones Sanitarias"},{"name":"Spanish National Institute of Health","award":["FIS 06\/0453"],"award-info":[{"award-number":["FIS 06\/0453"]}]},{"name":"Instituto de Salud Carlos III of the Spanish National Institute of Health","award":["FI07\/00054"],"award-info":[{"award-number":["FI07\/00054"]}]},{"name":"Spanish Ministry of Science and Innovation and Blood and Tissue Bank","award":["PTQ-09-02-017050"],"award-info":[{"award-number":["PTQ-09-02-017050"]}]},{"name":"F.E.B.","award":["CES 07\/015"],"award-info":[{"award-number":["CES 07\/015"]}]},{"name":"Instituto de Salud Carlos III and Direcci\u00f3 d\u02c8Estrat\u00e8gia i Coordinaci\u00f3"},{"name":"Health Department of the Catalan government"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2012,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Involvement of \u03b3-secretase-mediated mechanisms, including the Notch pathway, in the cell-contact dependent communication between human DC subsets.<\/jats:p>\n               <jats:p>Cooperative events between DC subsets involve cell contact and soluble factors. Upon viral challenge, murine pDCs induce cDC cooperation through CD40-CD40L interactions and IL-15 secretion, whereas in humans, the same effect is mediated by IFN-\u03b1. Conversely, during bacterial infections, pDC maturation may be induced by activated cDCs, although no mechanisms had been described so far. Here, we investigate how human pDCs are \u201cconditioned\u201d by cDCs. Blood-borne DC subsets (cDCs and pDCs) were sorted from healthy donors. IL-3-maintained pDCs were cocultured with LPS-activated, poly (I:C)-activated, or control cDCs [cDCLPS, cDCP(I:C), cDCCTRL]. Coculture experiments showed that cDCLPS-conditioned pDCs up-regulated maturation markers, such as CD25 and CD86, whereas SNs contained higher amounts of IL-6 and CCL19 compared with control conditions. Gene-expression analyses on sorted cDCLPS or cDCP(I:C) conditioned pDCs confirmed the induction of several genes, including IL-6 and CCL19 and remarkably, several Notch target genes. Further studies using the \u03b3-secretase\/Notch inhibitor DAPT and soluble Notch ligands resulted in a significantly reduced expression of canonical Notch target genes in conditioned pDCs. DAPT treatment also hampered the secretion of CCL19 (but not of IL-6) by cDCLPS conditioned pDCs. These results reveal the involvement of \u03b3-secretase-mediated mechanisms, including the Notch pathway, in the cell contact-dependent communication between human DC subsets. The resulting partial activation of pDCs after encountering with mature cDCs endows pDCs with an accessory function that may contribute to T cell recruitment and activation.<\/jats:p>","DOI":"10.1189\/jlb.0911452","type":"journal-article","created":{"date-parts":[[2012,4,25]],"date-time":"2012-04-25T04:45:41Z","timestamp":1335329141000},"page":"133-143","source":"Crossref","is-referenced-by-count":7,"title":["TLR-activated conventional DCs promote \u03b3-secretase-mediated conditioning of plasmacytoid DCs"],"prefix":"10.1093","volume":"92","author":[{"given":"Bego\u00f1a","family":"P\u00e9rez-Cabezas","sequence":"first","affiliation":[{"name":"Laboratori d\u02c8Immunobiologia i Diagn\u00f2stic Molecular (LIRAD), Banc de Sang i Teixits (BST), Departament de Biologia Cel\u00b7lular, Fisiologia i Immunologia, Universitat Aut\u00f2noma de Barcelona, Institut d\u02c8Investigaci\u00f3 Germans Trias i Pujol , Badalona, Barcelona, Spain"}]},{"given":"Mar","family":"Naranjo-G\u00f3mez","sequence":"additional","affiliation":[{"name":"Laboratori d\u02c8Immunobiologia i Diagn\u00f2stic Molecular (LIRAD), Banc de Sang i Teixits (BST), Departament de Biologia Cel\u00b7lular, Fisiologia i Immunologia, Universitat Aut\u00f2noma de Barcelona, Institut d\u02c8Investigaci\u00f3 Germans Trias i Pujol , Badalona, Barcelona, Spain"}]},{"given":"Marta","family":"Ruiz-Riol","sequence":"additional","affiliation":[{"name":"Laboratori d\u02c8Immunobiologia i Diagn\u00f2stic Molecular (LIRAD), Banc de Sang i Teixits (BST), Departament de Biologia Cel\u00b7lular, Fisiologia i Immunologia, Universitat Aut\u00f2noma de Barcelona, Institut d\u02c8Investigaci\u00f3 Germans Trias i Pujol , Badalona, Barcelona, Spain"}]},{"given":"Patricia","family":"Bastos-Amador","sequence":"additional","affiliation":[{"name":"Laboratori d\u02c8Immunobiologia i Diagn\u00f2stic Molecular (LIRAD), Banc de Sang i Teixits (BST), Departament de Biologia Cel\u00b7lular, Fisiologia i Immunologia, Universitat Aut\u00f2noma de Barcelona, Institut d\u02c8Investigaci\u00f3 Germans Trias i Pujol , Badalona, Barcelona, Spain"}]},{"given":"Marco A","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Unitat de citometria, Institut d\u02c8investigaci\u00f3 Germans Trias i Pujol , Badalona, Barcelona, Spain"}]},{"given":"Francesc","family":"Carmona","sequence":"additional","affiliation":[{"name":"Department d\u02c8estad\u00edstica, Universitat de Barcelona , Barcelona, Spain"}]},{"given":"Fatima","family":"Nu\u00f1ez","sequence":"additional","affiliation":[{"name":"Unitat cient\u00edfico-t\u00e8cnica de suport, Institut d\u02c8Investigaci\u00f3 Vall d\u02c8Hebron , Barcelona, Spain"}]},{"given":"Ricardo","family":"Pujol-Borrell","sequence":"additional","affiliation":[{"name":"Laboratori d\u02c8Immunobiologia i Diagn\u00f2stic Molecular (LIRAD), Banc de Sang i Teixits (BST), Departament de Biologia Cel\u00b7lular, Fisiologia i Immunologia, Universitat Aut\u00f2noma de Barcelona, Institut d\u02c8Investigaci\u00f3 Germans Trias i Pujol , Badalona, Barcelona, Spain"}]},{"given":"Francesc E","family":"Borr\u00e0s","sequence":"additional","affiliation":[{"name":"Laboratori d\u02c8Immunobiologia i Diagn\u00f2stic Molecular (LIRAD), Banc de Sang i Teixits (BST), Departament de Biologia Cel\u00b7lular, Fisiologia i Immunologia, Universitat Aut\u00f2noma de Barcelona, Institut d\u02c8Investigaci\u00f3 Germans Trias i Pujol , Badalona, Barcelona, Spain"}]}],"member":"286","published-online":{"date-parts":[[2012,7,1]]},"reference":[{"key":"2023012312542357900_B1","doi-asserted-by":"crossref","first-page":"2769","DOI":"10.1002\/(SICI)1521-4141(199909)29:09<2769::AID-IMMU2769>3.0.CO;2-2","article-title":"Human peripheral blood contains two distinct lineages of dendritic cells","volume":"29","author":"Robinson","year":"1999","journal-title":"Eur. 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