{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:31:50Z","timestamp":1777667510988,"version":"3.51.4"},"reference-count":58,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2000,11,1]],"date-time":"2000-11-01T00:00:00Z","timestamp":973036800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hum Exp Toxicol"],"published-print":{"date-parts":[[2000,11]]},"abstract":"<jats:p>In this study we examined the effect of the aqueous extract of Thonningia sanguinea (T.S.) on 7-ethoxyresorufin 0- deethylase (EROD, CYPlAl), 7-pentoxyresorufin-dealkylase (PROD, CYP2B1\/2), 7-methoxyresorufin 0- demethylase (MROD, CYP1A2), aniline hydroxylase (aniline, CYP2E1), p-nitrophenol hydroxylase (PNPH, CYP2E1) and erythromycin N-demethylase (ERDM, CYP3A1) in rat liver in vitro and in vivo. Although T.S. extract increased ERDM activity in induced rat liver microsomes, it showed a dose-dependent inhibitory effectin vitro on other P450 monooxygenase activities particularly EROD and PROD, which are mediated primarily by CYPlAl and CYP2B1\/2, respectively. PROD, EROD and MROD activities were also decreased by 18%, 19% and 40%, respectively, in hepatic microsomes prepared from rats treated with T.S. extract for 3 days. Kinetic analysis of CYP activity of 3-methylchloranthrene-induced microsomes demonstrated that T.S. inhibited EROD and MROD activities by a noncompetitive and competitive mechanism, respectively. The analysis of alterations produced by T.S. on PROD kinetic parameters in phenobarbital-induced microsomes suggested that the inhibition is noncompetitive. Pretreatment of rats with T.S. prolonged pentobarbital and phenobarbital sleeping time; however, plasma phenobarbital concentration determined on awakening showed no significant difference between control and T.S.-treated rats. T.S. was also found to be a potent inhibitor of the liver cytosolic glutathione S-transferase. These data suggest that selective modulation of CYP isoenzymes by T.S. might con-tribute to protection of the liver from xenobiotic-induced intoxication or to alteration of the action of drug (s) conco-mitantly administered besides its antioxidative properties.<\/jats:p>","DOI":"10.1191\/096032700667732543","type":"journal-article","created":{"date-parts":[[2003,4,3]],"date-time":"2003-04-03T19:38:43Z","timestamp":1049398723000},"page":"623-631","source":"Crossref","is-referenced-by-count":23,"title":["Inhibitory effects of the medicinal herb, Thonningia san gunea, on liver drug                 metabolizing enzymes of rats"],"prefix":"10.1177","volume":"19","author":[{"given":"M A","family":"Gyamfi","sequence":"first","affiliation":[{"name":"Laboratory of Physiology and Pharmacology, School of Health Sciences,                         Faculty of Medicine, University of the Ryukyus, Nishihara, Okinawa 903-0215, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Hokama","sequence":"additional","affiliation":[{"name":"Department of Hospital Pharmacy, Faculty of Medicine, University of the                         Ryukyus, Nishihara, Okinawa 903-0215, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Oppong-Boachie","sequence":"additional","affiliation":[{"name":"Centre for Scientific Research into Plant Medicine, P.O. 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