{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:35:47Z","timestamp":1777667747228,"version":"3.51.4"},"reference-count":31,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2001,2,1]],"date-time":"2001-02-01T00:00:00Z","timestamp":980985600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hum Exp Toxicol"],"published-print":{"date-parts":[[2001,2]]},"abstract":"<jats:p>\n                    Progressive renal dysfunction in 5\/6 nephrectomized (NX) rats can be physiologically divided into three stages, coinciding with morphological stages, after definition of physiological parameters for identification of stage. Now, for the establishment of a toxicity screening approach using 5\/6 NX rats, our concept, \u201cDifferential toxicity synchronized with renal dysfunction process could be identified using 5\/6 NX rats\u201d was examined by dosing gentamicin. Firstly, electrophoretic fractional changes of urinary proteins during gentamicin treatment were clarified with determination of amino acid sequences and the three differential features were proven, revealing the unpredictable depression of urinary albumin with progression of the stages in NX rats. Secondly, marked elevation of urinary lactate dehydrogenase (LDH) and glucose (GLU) was evident, indicating the intensified hypoxic conditions and glycolysis in tubular cells synchronized with increased tubular damage. Thirdly, these transit metabolic changes were proven as intensive cause for the advancement of renal dysfunction by the reduction of FR\n                    <jats:sub>electrolytes and water<\/jats:sub>\n                    at the end of each dosing period. These results indicate that toxicity studies of newly developed drugs using 5\/6 NX rats have potentiality prior to clinical dosing to the patients.\n                  <\/jats:p>","DOI":"10.1191\/096032701666840048","type":"journal-article","created":{"date-parts":[[2003,4,3]],"date-time":"2003-04-03T19:38:43Z","timestamp":1049398723000},"page":"100-110","source":"Crossref","is-referenced-by-count":0,"title":["Differential toxicity expression of gentamicin in five-sixths nephrectomized                 rats assigned to three progressive stages of renal dysfunction \u2014                 establishment of a new screening approach"],"prefix":"10.1177","volume":"20","author":[{"given":"K","family":"Barata","sequence":"first","affiliation":[{"name":"The United Graduate School of Veterinary Science, Yamaguchi University,                         1677-1 Yoshida, Yamaguchi 753-0000, Japan; 147-6 Kamitamari, Tamari-mura,                         Niiharigun, Ibaraki 311-3436, Japan; Kyowa Hakko Kogyo, 6-1, Ohthemachi,                         1-Chome, Chiyoda-ku, Tokyo 100-8185, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Pathology, Sasaki Institute, 2-2 Kanda-Surugadai,                         Chiyoda-ku, Tokyo 101-0062, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Hokao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Imai","sequence":"additional","affiliation":[{"name":"Imamichi Institute for Animal Reproduction, Ibaraki, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Takahashi","sequence":"additional","affiliation":[{"name":"faculty of Agriculture Yamaguchi University, Yamaguchi, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Harada","sequence":"additional","affiliation":[{"name":"faculty of Agriculture Tottori University, Tottori, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Maekawa","sequence":"additional","affiliation":[{"name":"Department of Pathology, Sasaki Institute, 2-2 Kanda-Surugadai,                         Chiyoda-ku, Tokyo 101-0062, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2001,2,1]]},"reference":[{"key":"atypb1","volume-title":"CRC Handbook of Animal Models of Renal Failure","author":"Reviere JE","year":"1985"},{"key":"atypb2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-364930-0.50009-9"},{"key":"atypb3","doi-asserted-by":"publisher","DOI":"10.2131\/jts.23.5_433"},{"key":"atypb4","first-page":"95","volume":"79","author":"Shimamura T","year":"1975","journal-title":"Am J Pathol"},{"key":"atypb5","first-page":"500","volume":"100","author":"Elliott WC","year":"1982","journal-title":"J Lab Clin Med"},{"key":"atypb6","first-page":"513","volume":"100","author":"Elliott WC","year":"1982","journal-title":"J Lab Clin Med"},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(90)90126-9"},{"key":"atypb8","volume-title":"Methods in Renal Toxicology","author":"Rudolf KZ","year":"1996"},{"key":"atypb9","doi-asserted-by":"publisher","DOI":"10.1159\/000414809"},{"key":"atypb10","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1992.tb04046.x"},{"key":"atypb11","first-page":"83","volume":"101","author":"Faraj AH","year":"1993","journal-title":"AMPIS"},{"key":"atypb12","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"atypb13","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"atypb14","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/140.4.576"},{"key":"atypb15","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/13.suppl_A.9"},{"key":"atypb16","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/38.5.885"},{"key":"atypb17","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/9.4.297"},{"key":"atypb18","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1972.03190340043010"},{"key":"atypb19","first-page":"1","volume":"11","author":"Hatala M","year":"1975","journal-title":"Int J Clin Pharmacol Ther Toxicol"},{"key":"atypb20","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-74-2-192"},{"key":"atypb21","first-page":"29","volume":"47","author":"Jung K","year":"1994","journal-title":"Kidney Int"},{"key":"atypb22","doi-asserted-by":"publisher","DOI":"10.1007\/s002040050398"},{"key":"atypb23","doi-asserted-by":"publisher","DOI":"10.3109\/10408449109019572"},{"key":"atypb24","first-page":"265","volume":"3","author":"Luft FC","year":"1980","journal-title":"Renal Physiol (Basel)"},{"key":"atypb25","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.1998.00797.x"},{"key":"atypb26","first-page":"121","volume":"24","author":"Cuppage FE","year":"1977","journal-title":"Virchows Arch B: Cell Pathol"},{"key":"atypb27","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1996.187"},{"key":"atypb28","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.1999.00238.x"},{"key":"atypb29","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1994.132"},{"key":"atypb30","volume-title":"The Text of Physiology","author":"Ohchi R","year":"1999"},{"key":"atypb31","doi-asserted-by":"publisher","DOI":"10.1159\/000008570"}],"container-title":["Human &amp; Experimental Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1191\/096032701666840048","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1191\/096032701666840048","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T07:47:46Z","timestamp":1777448866000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1191\/096032701666840048"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,2]]},"references-count":31,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2001,2]]}},"alternative-id":["10.1191\/096032701666840048"],"URL":"https:\/\/doi.org\/10.1191\/096032701666840048","relation":{},"ISSN":["0960-3271","1477-0903"],"issn-type":[{"value":"0960-3271","type":"print"},{"value":"1477-0903","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,2]]}}}