{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T20:29:48Z","timestamp":1777667388178,"version":"3.51.4"},"reference-count":23,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[1999,11,1]],"date-time":"1999-11-01T00:00:00Z","timestamp":941414400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hum Exp Toxicol"],"published-print":{"date-parts":[[1999,11]]},"abstract":"<jats:p>By-products of lipoperoxidation reactions may be associated with the genesis or the progression of several diseases as arteriosclerosis, diabetes and cancer, among many others. Acrolein, at first a widely distributed environmental pollutant, is currently known as a compound capable of being generated as a result of metabolic reactions within biological systems, highly toxic and the most electrophilic of the a, b-unsaturated aldehydes formed during lipoperoxidation. In the present study:<\/jats:p>\n                  <jats:p>1 The separation of acrolein and malondialdehyde was achieved at alkaline pH with the use of high voltage capillary electrophoresis in uncoated fused-silica capillaries. 2 It was demonstrated how the oxidation of fatty acids (arachidonic\/linoleic) with ozone generates, in dose-dependent form, acrolein as one of the by-products of the lipoperoxidation process. The oxidation of open human erythrocyte membranes with ozone also generated acrolein. 3 After aldolic condensation, aldol-acrolein derivative has a positive reaction with 2-thiobarbituric acid (TBA) and shows a maximum absorption at 498 nm. This novel characteristic is used in its identification after the separation of the by-products. 4 It is possible to suggest that in the classic reaction of the denominated thiobarbituric acid reactive substances (TBARS), when used as an indicator of the degree of peroxidation in biological systems, a portion of acrolein could be present but dwarfed by the TBAMDA adduct.<\/jats:p>","DOI":"10.1191\/096032799678839527","type":"journal-article","created":{"date-parts":[[2003,4,3]],"date-time":"2003-04-03T19:38:43Z","timestamp":1049398723000},"page":"677-682","source":"Crossref","is-referenced-by-count":13,"title":["Identification of acrolein from the ozone oxidation of unsaturated fatty acids"],"prefix":"10.1177","volume":"18","author":[{"given":"R","family":"Medina-Navarro","sequence":"first","affiliation":[{"name":"Specialties Hospital, Medical Research Unit in Biochemistry, Mexican                         Institute of Social Security, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Mercado-Pichardo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O","family":"Her\u0144ndez-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Metropolitan Autonomous University, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J J","family":"Hicks","sequence":"additional","affiliation":[{"name":"Juarez Mexico City Hospital, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1999,11,1]]},"reference":[{"key":"atypb1","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(91)90192-6"},{"key":"atypb2","first-page":"99","volume":"13","author":"Li L","year":"1998","journal-title":"Review of Environmental Health"},{"key":"atypb3","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.26.16058"},{"key":"atypb4","doi-asserted-by":"crossref","first-page":"424","DOI":"10.1172\/JCI119176","volume":"99","author":"Anderson MH","year":"1997","journal-title":"Journal of Clinical Investigation"},{"key":"atypb5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.9.4882"},{"key":"atypb6","doi-asserted-by":"publisher","DOI":"10.1006\/taap.1997.8189"},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.1016\/S0024-3205(99)80001-0"},{"key":"atypb8","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-008X(88)80008-5"},{"key":"atypb9","doi-asserted-by":"publisher","DOI":"10.1016\/1357-2725(95)00130-1"},{"key":"atypb10","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9150(98)00142-7"},{"key":"atypb11","doi-asserted-by":"publisher","DOI":"10.1021\/ac00276a032"},{"key":"atypb12","first-page":"1086","volume":"87","author":"Uchida K","year":"1993","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"atypb13","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(90)90131-2"},{"key":"atypb14","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(88)80330-9"},{"key":"atypb15","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(74)31019-1"},{"key":"atypb16","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(84)90152-8"},{"key":"atypb17","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(81)90142-9"},{"key":"atypb18","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(84)05036-9"},{"key":"atypb19","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2697(79)80033-0"},{"key":"atypb20","doi-asserted-by":"publisher","DOI":"10.1042\/bj2080129"},{"key":"atypb21","volume-title":"Laboratory and Chemical Safety Summaries","year":"1995"},{"key":"atypb22","first-page":"95","volume":"114","author":"Chilarducci DP","year":"1995","journal-title":"Review of Environmental Toxicology"},{"key":"atypb23","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(86)90016-0"}],"container-title":["Human &amp; Experimental Toxicology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1191\/096032799678839527","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1191\/096032799678839527","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T07:46:34Z","timestamp":1777448794000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1191\/096032799678839527"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,11]]},"references-count":23,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1999,11]]}},"alternative-id":["10.1191\/096032799678839527"],"URL":"https:\/\/doi.org\/10.1191\/096032799678839527","relation":{},"ISSN":["0960-3271","1477-0903"],"issn-type":[{"value":"0960-3271","type":"print"},{"value":"1477-0903","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,11]]}}}