{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T18:47:06Z","timestamp":1768589226570,"version":"3.49.0"},"reference-count":44,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2003,8,1]],"date-time":"2003-08-01T00:00:00Z","timestamp":1059696000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lupus"],"published-print":{"date-parts":[[2003,8]]},"abstract":"<jats:p>The objective was to determine the sensitivity and specificity of an automated                 multiparameter line immunoassay system compared with other techniques for the                 identification of autoantibodies in rheumatic diseases. We studied sera from 90                 patients. Anti-U1RNP, anti-Sm, anti-Ro\/SS-A, anti-La\/SS-B, anti-Jo 1 and anti-Scl 70                 antibodies were identified by counterimmunoelectrophoresis(CIE) techniques,                 enzyme-linked immunosorbent assay (ELISA), immunoblotting (IB) using extracts of                 rabbit thymus and human placenta, and an automated multiparameter line immunoassay                 system (INNO-LIA ANA UPDATE K-1090) that detects nine different antibodies                 simultaneously (anti-U1RNP, anti-Sm, anti-Ro\/SS-A, anti-La\/SS-B, anti-Scl 70,                 anti-Jo 1, anticentromere, antihistone, and antiribosomal P protein). The line                 immunoassay system equaled or surpassed the other techniques in the identification                 of anti-Sm, anti-La\/SS-B, anti-Jo 1 and anti-Scl 70 antibodies (sensitivity 100%,                 specificity 94-100%) and was similarly effective in the case of anti-U1RNP                 (sensitivity 87.5%, specificity 93.9%) and anti-Ro\/SS-A (sensitivity 91.4%,                 specificity 87.2%) antibodies. In addition, this technique detected more 52 and 60                 kD anti-Ro\/SS-A sera than IB. Nine antibodies can be detected with this method at a                 cost of 25.38\u20ac per serum sample. In five hours, 19 sera can be                 studied.The approximatecost of detecting these nine antibodieswith an automatedELISA                 system would be 28.93\u20ac, which allows 10 sera to be studied in four                 hours. In conclusion, the automated multiparameter line immunoassay system is a                 valid method for the detection of autoantibodies in rheumatic diseases. Its most                 notable advantages are automated simultaneous detection of several autoantibodies in                 the same serum and its lower cost compared with ELISA techniques.<\/jats:p>","DOI":"10.1191\/0961203303lu439oa","type":"journal-article","created":{"date-parts":[[2003,11,12]],"date-time":"2003-11-12T07:45:16Z","timestamp":1068623116000},"page":"623-629","source":"Crossref","is-referenced-by-count":32,"title":["Simultaneous identification of various antinuclear antibodies using an                 automated multiparameter line immunoassay system"],"prefix":"10.1177","volume":"12","author":[{"given":"F J","family":"L\u00f3pez-Longo","sequence":"first","affiliation":[{"name":"Service of Rheumatology, Hospital General Universitario                         \u2018Gregorio Mara\u00f1\u00f3n\u2019, Universidad                         Complutense of Madrid, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Rodr\u00edguez-Mahou","sequence":"additional","affiliation":[{"name":"Service of Immunology, Hospital General Universitario \u2018Gregorio                         Mara\u00f1\u00f3n\u2019, Universidad Complutense of Madrid,                         Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Escalona-Monge","sequence":"additional","affiliation":[{"name":"Experimental Surgery and Medicine Unit, Hospital General Universitario                         \u2018Gregorio Mara\u00f1\u00f3n\u2019, Universidad                         Complutense of Madrid, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C M","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Service of Rheumatology, Hospital General Universitario                         \u2018Gregorio Mara\u00f1\u00f3n\u2019, Universidad                         Complutense of Madrid, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Monteagudo","sequence":"additional","affiliation":[{"name":"Service of Rheumatology, Hospital General Universitario                         \u2018Gregorio Mara\u00f1\u00f3n\u2019, Universidad                         Complutense of Madrid, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Carre\u00f1o","sequence":"additional","affiliation":[{"name":"Service of Rheumatology, Hospital General Universitario                         \u2018Gregorio Mara\u00f1\u00f3n\u2019, Universidad                         Complutense of Madrid, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2003,8]]},"reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780400909"},{"key":"e_1_2_1_2_1","first-page":"341","article-title":"Antinuclear antibodies","volume":"23","author":"L\u00f3pez-Longo FJ.","year":"1996","unstructured":"L\u00f3pez-Longo FJ. 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