{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,2]],"date-time":"2025-03-02T05:28:59Z","timestamp":1740893339945,"version":"3.38.0"},"reference-count":29,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[1998,3,1]],"date-time":"1998-03-01T00:00:00Z","timestamp":888710400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lupus"],"published-print":{"date-parts":[[1998,3]]},"abstract":"<jats:p> The ability of affinity purified anti-52 kDa Ro\/SSA antibody from patients without obstetric history of neonatal lupus to cause heart block using an experimental model was investigated. IgG-enriched fractions from sera of 20 systemic lupus erythematosus (SLE) and one Sj\u00f6gren's syndrome (SS) all positives for anti-Ro\/SSA antibodies as detected by CIE, were perfused on isolated whole rabbit hearts. Only six (29%) samples induced A-V block, five of them presenting low anti-Ro\/SSA titre. All of them recognized the 52 kDa isoform on ELISA whereas only one had a concomitant binding to the 60 kDa protein. Moreover, affinity purified antibodies from two sera previously known to induce A-V block were obtained by affinity chromatography using a column containing the full-length 52 kDa Ro\/SSA fusion protein. Paired eluate and effluent devoid of anti-52 kDa activity from the same patient were individually perfused in whole hearts. The ability to cause cardiac blockade was restricted to the affinity anti-52 kDa eluates. In addition, anti-52 kDa eluates from three IgG fractions that primarily failed to induce blockade remained ineffective. The present study has added to our knowledge that affinity anti-52 kDa Ro\/SSA antibodies from mothers with healthy infants are capable of causing in vitro cardiac conduction disturbances. A prospective follow up of these patients will better delineate the clinical usefulness of this experimental model. <\/jats:p>","DOI":"10.1191\/096120398678919895","type":"journal-article","created":{"date-parts":[[2003,11,12]],"date-time":"2003-11-12T12:45:16Z","timestamp":1068641116000},"page":"141-147","source":"Crossref","is-referenced-by-count":25,"title":["Induction of in vitro heart block is not restricted to affinity purified                 anti-52 kDa Ro\/SSA antibody from mothers of children with neonatal lupus"],"prefix":"10.1177","volume":"7","author":[{"given":"V ST","family":"Viana","sequence":"first","affiliation":[{"name":"Division of Rheumatic Diseases, University of S\u00e3o Paulo, SP                         01246-903 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Garcia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J HM","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio                         de Janeiro, RJ 21949-900 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K B","family":"Elkon","sequence":"additional","affiliation":[{"name":"Hospital for Special Surgery, Cornell University, NY 10072"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Brot","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biology, Roche Center, NJ 07110"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A C","family":"Campos de Carvalho","sequence":"additional","affiliation":[{"name":"Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio                         de Janeiro, RJ 21949-900 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