{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T17:34:07Z","timestamp":1760204047264,"version":"3.38.0"},"reference-count":14,"publisher":"SAGE Publications","issue":"2-6","license":[{"start":{"date-parts":[[2003,3,1]],"date-time":"2003-03-01T00:00:00Z","timestamp":1046476800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Toxicol Ind Health"],"published-print":{"date-parts":[[2003,3]]},"abstract":"<jats:p> To investigate the early visceral distribution of mercury (Hg), we have intraperitoneally injected a lethal dose of HgCl<jats:sub>2<\/jats:sub> that killed BALB\/c mice within 2-4 min. Scanning electron microscopy coupled with X-ray microanalysis (SEM-XRM) was used to detect and quantify Hg in situ in different organs. The highest density of Hg was seen in the liver (60.99 \u00b124.9 Hg particles per mm<jats:sup>2<\/jats:sup> of tissue); this density was three and six times higher than those of renal or splenic Hg, respectively. Hg was scarce in the lungs and absent in the brain. Considering the relative weights of mouse viscera, our quantitative data show that the liver captured 89% of the visceral Hg; the kidneys captured 8.5% and the spleen just 1.7%. SEM-XRM revealed that most of the visceral Hg was associated with resident macrophages, a few Hg dots being detected on the surface of erythrocytes. We conclude that: (i) most intraperitoneally injected Hg was captured by liver Kupffer cells within minutes of injection; (ii) a 10-fold lower density of Hg particles was observed in the kidneys, and a 50-fold lower deposition of Hg was found in the spleen; (iii) SEM-XRM is an adequate method to quantify microparticles of Hg in tissues and cells. <\/jats:p>","DOI":"10.1191\/0748233703th175oa","type":"journal-article","created":{"date-parts":[[2004,12,13]],"date-time":"2004-12-13T23:27:02Z","timestamp":1102980422000},"page":"55-61","source":"Crossref","is-referenced-by-count":5,"title":["Quantification of particles of lethal mercury in mouse viscera: high-resolution study of mercury in cells and tissues"],"prefix":"10.1177","volume":"19","author":[{"given":"Elisabete M","family":"Cunha","sequence":"first","affiliation":[{"name":"Department of Anatomy, ICBAS (Abel Salazar Institute for Biomedical Sciences), UMIB (Unit for Multidisciplinary Investigation in Biomedicine), and IBMC (Institute for Cellular and Molecular Biology), University of Porto, 4099-003 Porto, Portugal,"}]},{"given":"D","family":"Cherdwongcharoensuk","sequence":"additional","affiliation":[{"name":"Department of Anatomy, ICBAS (Abel Salazar Institute for Biomedical Sciences), UMIB (Unit for Multidisciplinary Investigation in Biomedicine), and IBMC (Institute for Cellular and Molecular Biology), University of Porto, 4099-003 Porto, Portugal"}]},{"given":"Artur P","family":"\u00c1guas","sequence":"additional","affiliation":[{"name":"Department of Anatomy, ICBAS (Abel Salazar Institute for Biomedical Sciences), UMIB (Unit for Multidisciplinary Investigation in Biomedicine), and IBMC (Institute for Cellular and Molecular Biology), University of Porto, 4099-003 Porto, Portugal"}]}],"member":"179","published-online":{"date-parts":[[2003,3,1]]},"reference":[{"volume-title":"Toxicological Profile for Mercury","year":"2001","author":"Agency for Toxic Substance and Disease Registry (ATSDR)","key":"atypb1"},{"key":"atypb2","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001900108"},{"key":"atypb3","doi-asserted-by":"publisher","DOI":"10.1191\/0748233702th161oa"},{"key":"atypb4","first-page":"1291","volume":"8","author":"Clarkson, T. 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