{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T11:34:57Z","timestamp":1788176097066,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,1,24]],"date-time":"2006-01-24T00:00:00Z","timestamp":1138060800000},"content-version":"vor","delay-in-days":815,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2003,11]]},"abstract":"<jats:p><jats:bold>A<jats:sc>bstract<\/jats:sc>: <\/jats:bold> Achieving normoglycemia is the goal of diabetes therapy. Potentially, there are many ways to achieve this goal, including transplantation of cells exhibiting glucose\u2010responsive insulin secretion. However, to be applicable to the large number of people who might benefit from \u03b2 cell replacement, an unlimited supply of \u03b2 cells must be found. To address this problem, we have been developing cell lines from the human endocrine pancreas. In one case, a cell line, \u03b2lox5, has been developed from human islets that can be induced under some circumstances to differentiate into functional \u03b2 cells exhibiting appropriate glucose\u2010responsive insulin secretion. Inducing differentiation is complex, requiring the activation of multiple signaling pathways, including those downstream of those involved in cell\u2010cell contact and the glucagon\u2010like peptide\u20101 receptor. In addition, transfer of the PDX\u20101 gene is also necessary to render the cells competent for differentiation. However, it is clear that many other genes are involved in maintaining the commitment of \u03b2lox5 cells towards the \u03b2 cell lineage. Understanding the complement of genes required to establish and maintain a \u03b2 cell lineage commitment would be enormously helpful in efforts to develop a cell line that can be used for \u03b2 cell replacement therapies. Here, we provide further information on the characteristics of cell lines that we have developed from the human pancreas that are relevant to the development of a \u03b2 cell replacement therapy for diabetes.<\/jats:p>","DOI":"10.1196\/annals.1288.015","type":"journal-article","created":{"date-parts":[[2004,1,12]],"date-time":"2004-01-12T17:32:48Z","timestamp":1073928768000},"page":"138-147","source":"Crossref","is-referenced-by-count":16,"title":["Cell\u2010Based Therapies for Diabetes: Progress towards a Transplantable Human \u03b2 Cell Line"],"prefix":"10.1111","volume":"1005","author":[{"given":"PAMELA","family":"ITKIN\u2010ANSARI","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"IFAT","family":"GERON","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ERGENG","family":"HAO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"CARLA","family":"DEMETERCO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"BJORN","family":"TYRBERG","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FRED","family":"LEVINE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,7,7]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200007273430401"},{"key":"e_1_2_5_3_2","doi-asserted-by":"crossref","first-page":"D175","DOI":"10.2741\/demeter","article-title":"Gene therapy for diabetes","volume":"6","author":"Demeterco C.","year":"2001","journal-title":"Front. 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