{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T07:49:19Z","timestamp":1695628159544},"reference-count":12,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,1,24]],"date-time":"2006-01-24T00:00:00Z","timestamp":1138060800000},"content-version":"vor","delay-in-days":785,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2003,12]]},"abstract":"<jats:p><jats:bold>A<jats:sc>bstract<\/jats:sc>: <\/jats:bold> Pharmacological characterization is described for a human imidazoline binding site (I\u2010site) labeled by [<jats:sup>3<\/jats:sup>H]clonidine using standard autoradiographic method. Under conditions that mask a<jats:sub>2<\/jats:sub>\u2010adrenergic sites, only a single high affinity site was observed in human caudate and blood platelet sections. Affinity constants (K<jats:sub>i<\/jats:sub>) were highly correlated between the two tissues (<jats:italic>r<\/jats:italic> 5 0.90, <jats:italic>P<\/jats:italic> 5 0.0003). This site is dissimilar to classical I<jats:sub>1<\/jats:sub> and I<jats:sub>2<\/jats:sub> sites, even though both tissues possess abundant I<jats:sub>1<\/jats:sub> and I<jats:sub>2<\/jats:sub> sites by filtration binding methods. It is suggested that the isotonic buffer conditions inherent to the procedure alter drug affinities to the classical I<jats:sub>1<\/jats:sub> site.<\/jats:p>","DOI":"10.1196\/annals.1304.038","type":"journal-article","created":{"date-parts":[[2004,3,17]],"date-time":"2004-03-17T23:23:23Z","timestamp":1079565803000},"page":"296-301","source":"Crossref","is-referenced-by-count":0,"title":["Atypical [<sup>3<\/sup>H]Clonidine Binding Sites in Human Caudate and Platelets on Cryostat\u2010cut Sections"],"prefix":"10.1111","volume":"1009","author":[{"given":"H","family":"ZHU","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"HALARIS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JE","family":"PILETZ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,1,24]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1995.tb32388.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1995.tb32402.x"},{"key":"e_1_2_6_4_2","first-page":"1493","article-title":"Nonadrenergic imidazoline binding sites on human platelets","volume":"267","author":"Piletz J.E.","year":"1993","journal-title":"J. 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Pharmacol."}],"container-title":["Annals of the New York Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1196%2Fannals.1304.038","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1196\/annals.1304.038","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T07:18:07Z","timestamp":1695626287000},"score":1,"resource":{"primary":{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/10.1196\/annals.1304.038"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,12]]},"references-count":12,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2003,12]]}},"alternative-id":["10.1196\/annals.1304.038"],"URL":"https:\/\/doi.org\/10.1196\/annals.1304.038","archive":["Portico"],"relation":{},"ISSN":["0077-8923","1749-6632"],"issn-type":[{"value":"0077-8923","type":"print"},{"value":"1749-6632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,12]]}}}