{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T07:54:36Z","timestamp":1695628476728},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,1,12]],"date-time":"2006-01-12T00:00:00Z","timestamp":1137024000000},"content-version":"vor","delay-in-days":468,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:p><jats:bold>A<jats:sc>bstract<\/jats:sc>: <\/jats:bold> The present study was designed to immunochemically identify a coronin\u2010like protein in <jats:italic>Babesia bovis, B. bigemina, B. divergens<\/jats:italic>, and <jats:italic>B. canis<\/jats:italic>. A 2\u2010kbp cDNA insert of <jats:italic>B. bovis<\/jats:italic> carried by plasmid BvN9 was sequenced by the dideoxichain\u2010termination method on both strands. The cDNA insert contained a 1719\u2010bp long open reading frame coding for a deduced protein sequence of 61.7 kDa. Sequence analysis using the PSI\u2010BLAST program revealed about 30% protein sequence identity with a coronin\u2010like protein of <jats:italic>Plasmodium falciparum<\/jats:italic>. The encoding sequence of the cDNA insert lacking 70 amino acids at the N\u2010terminal was subcloned in frame into pGEX 4T\u20103 to produce a recombinant glutathione <jats:italic>S<\/jats:italic>\u2010transferase (GST)\u2010pBv fusion protein. Polyclonal antibodies prepared in rabbits immunized with the purified GST\u2010fusion protein recognized a <jats:italic>Babesia<\/jats:italic>\u2010specific component of approximately 60 kDa by immunoprecipitation with [<jats:sup>35<\/jats:sup>S]methionine\u2010labeled parasites. However, two molecules with relative sizes of 60 and 70 kDa were recognized in <jats:italic>Babesia<\/jats:italic>\u2010infected erythrocyte extracts by immunobloting analysis. The 70\u2010kDa component was apparently of host erythrocyte origin. In an indirect fluorescent antibody test, the rabbit serum strongly reacted with the merozoite stage of the four <jats:italic>Babesia<\/jats:italic> species, but also, although weakly, with the host erythrocyte. A cosedimentation assay performed with GST\u2010pBv fusion protein and exogenous actin from rabbit liver showed that the GST\u2010pBv fusion protein, but not the GST protein, was associated to actin. From these results, we conclude that the protein present in the four <jats:italic>Babesia<\/jats:italic> species analyzed here may be considered as a novel coronin\u2010like, actin\u2010binding protein.<\/jats:p>","DOI":"10.1196\/annals.1307.017","type":"journal-article","created":{"date-parts":[[2004,12,16]],"date-time":"2004-12-16T23:57:42Z","timestamp":1103241462000},"page":"125-138","source":"Crossref","is-referenced-by-count":9,"title":["Identification of a Coronin\u2010Like Protein in <i>Babesia<\/i> Species"],"prefix":"10.1111","volume":"1026","author":[{"given":"JULIO V.","family":"FIGUEROA","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ERIC","family":"PRECIGOUT","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"BERNARD","family":"CARCY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ANDR\u00c9","family":"GORENFLOT","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,1,12]]},"reference":[{"key":"e_1_2_5_2_2","unstructured":"McCosker P.J. 1981. 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