{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T20:03:12Z","timestamp":1792008192459,"version":"4.4.0"},"reference-count":4,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,1,12]],"date-time":"2006-01-12T00:00:00Z","timestamp":1137024000000},"content-version":"vor","delay-in-days":407,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:p><jats:bold>A<jats:sc>bstract<\/jats:sc>: <\/jats:bold> Active T cell locomotion depends on efficient repeated cycles of integrin receptor\/ligand interactions mediating cell adhesion and detachment, intracellular signaling cascades orchestrating posttranslation modifications of interacting proteins, dynamic reassembly of participating cytoskeletal elements, and structural support of associated scaffolding molecules. Using an integrated approach based on novel cutting edge technologies of live cell imaging, cell transfection, proteomics, and nanotechnology, we provide here a detailed characterisation of crucial mechanisms involved in LFA\u20101 integrin\u2010mediated T cell migration. Polarization and phenotypic changes associated with LFA\u20101\u2010triggered T cell locomotion is largely dependent on the intact functioning of the microtubule cytoskeleton. Experiments utilizing 4\u2010D (3\u2010D over time) confocal live imaging of T cells, microinjected with fully functional constructs encoding protein kinase C beta (PKC\u2010\u03b2) isoenzyme tagged with enhanced green fluorescent protein (GFP), elucidate that LFA\u20101\u2010induced activation is associated with translocation of PKC\u2010\u03b2 to sites associated with centrosomes and tubulin cytoskeleton in locomotory T lymphocytes. We also provide here a characterization of a novel microfluidics\u2010based multichannel platform enabling detailed analysis of leukocyte adhesion and migration under regulated shear stress conditions. Using precision machined surfaces, we demonstrate that the substrate topography can influence the motile response of the two different T cell types in different ways, and this can be quantified in terms of specified motility parameters. Finally, using an original <jats:italic>in situ<\/jats:italic> immunoprecipitation method, in which LFA\u20101 antibodies are utilized to induce intracellular association of proteins in the cytoskeletal\/signaling complex, we demonstrate that this complex includes a number of structural and signaling proteins, which have been identified by 2\u2010D electrophoresis and MALDI\u2010TOF protein sequencing.<\/jats:p>","DOI":"10.1196\/annals.1322.035","type":"journal-article","created":{"date-parts":[[2005,1,13]],"date-time":"2005-01-13T20:08:46Z","timestamp":1105646926000},"page":"313-319","source":"Crossref","is-referenced-by-count":43,"title":["A Multidisciplinary Approach to the Study of T Cell Migration"],"prefix":"10.1111","volume":"1028","author":[{"given":"AIDEEN","family":"LONG","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SIOBHAN","family":"MITCHELL","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DMITRY","family":"KASHANIN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"VIVIENNE","family":"WILLIAMS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ADRIELE","family":"PRINA MELLO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"IGOR","family":"SHVETS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DERMOT","family":"KELLEHER","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YURI","family":"VOLKOV","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,1,12]]},"reference":[{"key":"e_1_2_9_2_2","doi-asserted-by":"crossref","first-page":"6487","DOI":"10.4049\/jimmunol.161.12.6487","article-title":"Inside the crawling T cell: leukocyte function\u2010associated antigen\u20101 cross\u2010linking is associated with microtubule\u2010directed translocation of protein kinase C isoenzymes beta(I) and delta","volume":"161","author":"Volkov Y.","year":"1998","journal-title":"J. Immunol"},{"key":"e_1_2_9_3_2","doi-asserted-by":"crossref","unstructured":"Volkov Y. A. Long S. McGrath et al.2001. Crucial importance of PKC\u2010beta(I) in LFA\u20101\u2010mediated locomotion of activated T cells. Nat. Immunol.2:508\u2013514","DOI":"10.1038\/88700"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1535-5535(04)00235-7"},{"key":"e_1_2_9_5_2","first-page":"1106","article-title":"Cell migration on topographical substrate","volume":"31","author":"Prina Mello A.","year":"2003","journal-title":"Measurement of the migration factors associated with two motile lymphoid cell types. Ann. Biomed. Eng"}],"container-title":["Annals of the New York Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1196%2Fannals.1322.035","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1196\/annals.1322.035","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T17:28:18Z","timestamp":1695662898000},"score":1,"resource":{"primary":{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/10.1196\/annals.1322.035"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,12]]},"references-count":4,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,12]]}},"alternative-id":["10.1196\/annals.1322.035"],"URL":"https:\/\/doi.org\/10.1196\/annals.1322.035","archive":["Portico"],"relation":{},"ISSN":["0077-8923","1749-6632"],"issn-type":[{"value":"0077-8923","type":"print"},{"value":"1749-6632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,12]]}}}