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Polyadenylation is essential for eukaryotic mRNA expression, and CstF\u201064 is a subunit of the CstF polyadenylation factor that is required for accurate polyadenylation. We discovered that there are two forms of the CstF\u201064 protein in mammalian male germ cells, one of which (CstF\u201064) is expressed in all tissues, the other of which (\u03c4CstF\u201064) is expressed only in male germ cells and in brain (albeit at significantly lower levels in the brain). Therefore, we were surprised to find that, using reverse transcription\u2010PCR, cDNA cloning, and RNA blot analyses, \u03c4CstF\u201064 mRNA was expressed at higher levels in brain than in testis. Also, \u03c4CstF\u201064 mRNA was expressed at lower but detectable levels in all tissues tested, including epididymis, heart, kidney, liver, lung, muscle, ovary, spleen, thymus, and uterus. These results suggest the hypothesis that \u03c4CstF\u201064 mRNA is regulated at the translational or post\u2010translational level.<\/jats:p>","DOI":"10.1196\/annals.1336.017","type":"journal-article","created":{"date-parts":[[2006,2,7]],"date-time":"2006-02-07T23:23:00Z","timestamp":1139354580000},"page":"163-172","source":"Crossref","is-referenced-by-count":10,"title":["The mRNA Encoding \u03c4CstF\u201064 Is Expressed Ubiquitously in Mouse Tissues"],"prefix":"10.1111","volume":"1061","author":[{"given":"ZANE","family":"HUBER","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ROBERTO R.","family":"MONAREZ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"BRINDA","family":"DASS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CLINTON C.","family":"MacDONALD","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,3,29]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(99)80048-9"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(99)80049-0"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.63.2.405-445.1999"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.4.12a.2112"},{"key":"e_1_2_7_6_2","doi-asserted-by":"crossref","first-page":"19768","DOI":"10.1016\/S0021-9258(18)55058-4","article-title":"Purification of the cleavage and polyadenylation factors involved in the 3\u2032\u2010processing of messenger RNA precursors","volume":"266","author":"Bienroth S.","year":"1991","journal-title":"J. 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