{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T03:08:56Z","timestamp":1762916936844},"reference-count":16,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,1,12]],"date-time":"2006-01-12T00:00:00Z","timestamp":1137024000000},"content-version":"vor","delay-in-days":407,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:p><jats:bold>A<jats:sc>bstract<\/jats:sc>: <\/jats:bold> The ability to measure proliferation of autoantigen\u2010specific T cells is critical for the evaluation of cellular immune function. Using a novel, sensitive, CFSE\u2010based assay, we were able to directly quantitate autoantigen\u2010specific CD4<jats:sup>+<\/jats:sup> T cell proliferation. However, peripheral blood cells from healthy, pre\u2010diabetic and diabetic donors exhibited overlap in responses to glutamic acid decarboxylase (GAD65) and proinsulin (PI). This indicates that autoantigen\u2010induced CD4<jats:sup>+<\/jats:sup> T cell proliferation in a functionally complex cell population may not discriminate disease in the general population. Clear discrimination was found between diabetic and healthy sibs, suggesting the need to standardize the genetic and environmental background. In addition, the ability of the CFSE assay to allow analysis of the phenotype and function of autoantigen\u2010responsive T cells may improve discrimination.<\/jats:p>","DOI":"10.1196\/annals.1337.003","type":"journal-article","created":{"date-parts":[[2005,2,8]],"date-time":"2005-02-08T00:28:29Z","timestamp":1107822509000},"page":"16-21","source":"Crossref","is-referenced-by-count":16,"title":["CD4<sup>+<\/sup> T Cell Proliferation in Response to GAD and Proinsulin in Healthy, Pre\u2010diabetic, and Diabetic Donors"],"prefix":"10.1111","volume":"1037","author":[{"given":"STUART I.","family":"MANNERING","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JESSICA S.","family":"MORRIS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"NATALIE L.","family":"STONE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KENT P.","family":"JENSEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PETER M.","family":"VAN ENDERT","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LEONARD C.","family":"HARRISON","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,1,12]]},"reference":[{"issue":"2","key":"e_1_2_5_2_2","first-page":"267","article-title":"Autoreactive T cell responses in insulin\u2010dependent (Type 1) diabetes mellitus","volume":"13","author":"Roep B.O.","year":"1999","journal-title":"Report of the first international workshop for standardization of T cell assays. 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