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The objective of the present study was to explore effects of 5\u2010FU on metabolism of ATP, 2,3\u2010BPG, and oxygen in erythrocytes and to relate these to the phenomenon of 5\u2010FU cardiotoxicity. We determined that 5\u2010FU induced rapid increase in O<jats:sub>2<\/jats:sub> consumption, which led to drastic changes in the metabolism of phosphate compounds in erythrocytes. Decrease in pO<jats:sub>2<\/jats:sub> provoked increase in production of 2,3\u2010BPG and subsequent deoxygenation of oxyHb to deoxyHb. However, the most important effect of 5\u2010FU on erythrocytes is severe decrease in the level of ATP. This could lead to a number of irreversible changes in erythrocyte structure and functioning, such as echinocytosis, increase in membrane fluidity, and non\u2010functioning of membrane ion pumps. All these changes affect normal functioning of erythrocytes, leading to difficulties in oxygen transport and insufficient supply of oxygen to the heart, and pointing to the importance of studying the effects of antineoplastic drugs on intracellular metabolism of erythrocytes.<\/jats:p>","DOI":"10.1196\/annals.1342.028","type":"journal-article","created":{"date-parts":[[2005,9,9]],"date-time":"2005-09-09T00:58:05Z","timestamp":1126227485000},"page":"311-320","source":"Crossref","is-referenced-by-count":10,"title":["<sup>31<\/sup>P NMR Spectroscopy and Polarographic Combined Study of Erythrocytes Treated with 5\u2010Fluorouracil: Cardiotoxicity\u2010Related Changes in ATP, 2,3\u2010BPG, and O<sub>2<\/sub> Metabolism"],"prefix":"10.1111","volume":"1048","author":[{"given":"IVAN","family":"SPASOJEVI\u0106","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JOANNA","family":"ZAKRZEWSKA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GORAN G.","family":"BA\u010cI\u0106","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,7,8]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-199957040-00003"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(75)91270-2"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-198916040-00002"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19880101)61:1<36::AID-CNCR2820610108>3.0.CO;2-6"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01724805"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1997.4003212.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.3109\/02841869509093643"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(96)00485-9"},{"key":"e_1_2_6_10_2","unstructured":"Spasojevic I.et al.2004. 5\u2010Fluorouracil induced cardiotoxicity. 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