{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T08:15:22Z","timestamp":1765959322339},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,3,29]],"date-time":"2006-03-29T00:00:00Z","timestamp":1143590400000},"content-version":"vor","delay-in-days":118,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:p><jats:bold>A<jats:sc>bstract<\/jats:sc>: <\/jats:bold> The gene fragments encoding outer membrane protein 1 (P1) and heat\u2010shock protein B (HspB) amplified from genomic DNA of <jats:italic>Coxiella burnetii<\/jats:italic> Xinqiao by PCR were inserted into prokaryotic expression vector pQE30 to construct recombinant expression plasmids pQE30\/<jats:italic>p1<\/jats:italic> and pQE30\/<jats:italic>hspB<\/jats:italic>, respectively. The <jats:italic>p1<\/jats:italic> fragment from pQE30\/<jats:italic>p1<\/jats:italic> was ligated with <jats:italic>hspB<\/jats:italic> of pQE30\/<jats:italic>hspB<\/jats:italic> to construct pQE30\/<jats:italic>p1\u2010hspB<\/jats:italic>. Recombinant proteins, P1, HspB, and P1\u2010HspB, were expressed in <jats:italic>Escherichia coli<\/jats:italic> cells transformed with pQE30\/<jats:italic>p1<\/jats:italic>, pQE30\/<jats:italic>hspB<\/jats:italic>, and pQE30\/<jats:italic>p1\u2010hspB<\/jats:italic>, respectively. The purified recombinant proteins and whole\u2010cell antigen (WCA) of <jats:italic>C. burnetii<\/jats:italic> were used to immunize BALB\/c mice. The antibody detection, T\u2010cell proliferation assay, and cytokine detection demonstrated that the animals immunized with P1\u2010HspB or WCA exhibited stronger humoral and cellular immune responses compared with animals immunized with P1 or HspB individually. Seven days after challenge of 10\u2010fold 50% infection dose of <jats:italic>C. burnetii<\/jats:italic>, mice were euthanized and their spleens were collected. The splenic weights of mice immunized with P1\u2010HspB or WCA were significantly lighter than that of mice immunized with P1 or HspB. By real\u2010time PCR assay, the coxiella loads of spleens of mice immunized with P1\u2010HspB or WCA were also significantly lower than that of mice immunized with P1 or HspB. The data from this study indicate that fusion antigen P1\u2010HspB is a good immunogen for eliciting immunoresponses against <jats:italic>C. burnetii<\/jats:italic>, and it may be a more suitable candidate for preparing subunit vaccine against Q fever.<\/jats:p>","DOI":"10.1196\/annals.1355.021","type":"journal-article","created":{"date-parts":[[2006,2,15]],"date-time":"2006-02-15T19:58:18Z","timestamp":1140033498000},"page":"130-142","source":"Crossref","is-referenced-by-count":33,"title":["Protective Immunity against Q Fever Induced with a Recombinant P1 Antigen Fused with HspB of <i>Coxiella burnetii<\/i>"],"prefix":"10.1111","volume":"1063","author":[{"given":"QINGFENG","family":"LI","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DONGSHENG","family":"NIU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"BOHAI","family":"WEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MEILING","family":"CHEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LING","family":"QIU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JINGBO","family":"ZHANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,3,29]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.4.518"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(03)00804-1"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.37.9.1733"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268800059458"},{"key":"e_1_2_6_6_2","doi-asserted-by":"crossref","first-page":"1251","DOI":"10.1128\/iai.61.4.1251-1258.1993","article-title":"Safety and immunogenicity in human volunteers of a chloroform\u2010methanol residue vaccine for Q fever","volume":"61","author":"Fries L.F.","year":"1993","journal-title":"Infect. 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