{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T16:15:17Z","timestamp":1759940117297},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,3,29]],"date-time":"2006-03-29T00:00:00Z","timestamp":1143590400000},"content-version":"vor","delay-in-days":118,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:p><jats:bold>A<jats:sc>bstract<\/jats:sc>: <\/jats:bold> Previous attempts to develop Q fever vaccines were less successful in that the vaccines caused unacceptable side effects or failed to be protective. In this study, we tested the efficacy of a mixture of eight recombinant <jats:italic>Coxiella burnetii<\/jats:italic> (<jats:italic>C. b.<\/jats:italic>) proteins in sublethal challenge infections with mice. Eight potential <jats:italic>C. b.<\/jats:italic> virulence genes (Omp, Pmm, HspB, Fbp, Orf410, Crc, CbMip, and MucZ) were overexpressed in <jats:italic>E. coli<\/jats:italic> as <jats:italic>his<\/jats:italic>\u2010tagged fusion proteins and partially purified. All recombinant proteins but rPmm proved to be antigenic in BALB\/c mice when administered as protein mixtures. For efficacy testing, mice were immunized with an adjuvanted mixture of the eight recombinant proteins and subsequently challenged intraperitoneally with the <jats:italic>C. b.<\/jats:italic> isolate Nine Mile RSA493 (1.8 \u00d7 10<jats:sup>8<\/jats:sup><jats:italic>C. b.<\/jats:italic>). Only animals vaccinated with the licensed Q fever vaccine Q\u2010Vax\u2122 (vaccination control) exhibited milder symptoms and minor gain of spleen and liver weights. In summary, clinical examinations and dissection of mice immunized with the eight recombinant <jats:italic>C. b.<\/jats:italic> proteins did not indicate a protective immune response after test infection.<\/jats:p>","DOI":"10.1196\/annals.1355.022","type":"journal-article","created":{"date-parts":[[2006,2,15]],"date-time":"2006-02-15T19:58:18Z","timestamp":1140033498000},"page":"143-148","source":"Crossref","is-referenced-by-count":12,"title":["Immunization Experiments with Recombinant <i>Coxiella burnetii<\/i> Proteins in a Murine Infection Model"],"prefix":"10.1111","volume":"1063","author":[{"given":"JUDITH","family":"TYCZKA","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SANDRA","family":"EBERLING","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GEORG","family":"BALJER","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,3,29]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.4.518"},{"key":"e_1_2_6_3_2","first-page":"71","article-title":"Vaccination against Q fever","volume":"47","author":"Smadel J.E.","year":"1948","journal-title":"Am. 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