{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T00:40:02Z","timestamp":1695688802648},"reference-count":5,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,3,29]],"date-time":"2006-03-29T00:00:00Z","timestamp":1143590400000},"content-version":"vor","delay-in-days":118,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:p><jats:bold>A<jats:sc>bstract<\/jats:sc>: <\/jats:bold> The Balb\/c mice were infected with <jats:italic>Coxiella burnetii<\/jats:italic> and samples of blood and major organs from the infected mice were collected on days 1 to 14 after infection. The DNAs extracted from the samples were detected by a developed real\u2010time quantitative PCR specific for <jats:italic>C. burnetii<\/jats:italic> and high loads of <jats:italic>C. burnetii<\/jats:italic> were found in spleens, livers, and lungs, particularly in spleens. The Balb\/c mice were immunized with whole cell antigen (WCA) of <jats:italic>C. burnetii<\/jats:italic> and coxiella loads in spleens of mice were assessed by the real\u2010time quantitative PCR on day 7 after challenge with <jats:italic>C. burnetii<\/jats:italic>. The analysis suggested that phase I whole cells were excellent immunogen that elicited complete protection against coxiella infection by two\u2010booster but not one\u2010booster immunization. The results suggest that the combination of Balb\/c model and the real\u2010time quantitative PCR assay is a reliable and sensitive way to evaluate the efficiency of vaccines against Q fever.<\/jats:p>","DOI":"10.1196\/annals.1355.027","type":"journal-article","created":{"date-parts":[[2006,2,15]],"date-time":"2006-02-15T19:58:18Z","timestamp":1140033498000},"page":"171-175","source":"Crossref","is-referenced-by-count":7,"title":["Balb\/c Mouse Model and Real\u2010Time Quantitative Polymerase Chain Reaction for Evaluation of the Immunoprotectivity against Q Fever"],"prefix":"10.1111","volume":"1063","author":[{"given":"JINGBO","family":"ZHANG","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"BOHAI","family":"WEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MEILING","family":"CHEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JUN","family":"ZHANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DONGSHENG","family":"NIU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,3,29]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1128\/CMR.12.4.518","article-title":"Q fever","volume":"12","author":"Raoult D.","year":"1999","journal-title":"Clin. 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