{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T17:03:51Z","timestamp":1758992631618,"version":"3.32.0"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,3,29]],"date-time":"2006-03-29T00:00:00Z","timestamp":1143590400000},"content-version":"vor","delay-in-days":118,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2005,12]]},"abstract":"<jats:p><jats:bold>A<jats:sc>bstract<\/jats:sc>:<\/jats:bold><jats:italic>Coxiella burnetii<\/jats:italic>, the etiological agent of Q fever, is a class B biodefense agent. We are continuing the momentum of discovery generated by the first<jats:italic>Coxiella<\/jats:italic>genome sequences by extending the breadth of genomics to include four additional heterogeneous<jats:italic>C. burnetii<\/jats:italic>strains. We are also sequencing the genome of<jats:italic>Rickettsiella grylli<\/jats:italic>, an intracellular parasite of grasshoppers and the closest known phylogenetic relative to the<jats:italic>Coxiella<\/jats:italic>group. These data will enable the investigation of fundamental questions about<jats:italic>Coxiella<\/jats:italic>pathogenicity and virulence as well as broader evolutionary questions about the transition to obligate intracellular life. Specifically, sequence comparisons will permit examination of genetic differences, allowing us to address key questions: What core genes are necessary for an obligate intracellular lifestyle and developmental cycle of the genus? What specific genetic determinants can be linked to virulence properties such as host preference, disease severity, and pathology (i.e., acute vs. chronic disease)? What are the frequencies of mutation and intragenomic recombination, and levels of genome reduction? What specific factors are relevant to colonization and virulence in human hosts (based on comparisons with<jats:italic>R. grylli<\/jats:italic>)? From a public health and biodefense perspective, exposure to different strains, either natural or due to illegitimate release, may have different outcomes. With extensive genomic\u2010level information from diverse strains, investigators can determine effective drug and vaccine targets and design methods to accurately type<jats:italic>Coxiella<\/jats:italic>based on a subset of genes, opening the way for cost\u2010effective targeted PCR\u2010 or antibody\u2010based tests.<\/jats:p>","DOI":"10.1196\/annals.1355.063","type":"journal-article","created":{"date-parts":[[2006,2,15]],"date-time":"2006-02-15T19:58:18Z","timestamp":1140033498000},"page":"442-450","source":"Crossref","is-referenced-by-count":38,"title":["Genome Analysis of<i>Coxiella burnetii<\/i>Species: Insights into Pathogenesis and Evolution and Implications for Biodefense"],"prefix":"10.1111","volume":"1063","author":[{"given":"REKHA","family":"SESHADRI","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JAMES","family":"SAMUEL","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,3,29]]},"reference":[{"key":"e_1_2_8_2_2","unstructured":"Anonymous. 2003. 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Portland OR."},{"key":"e_1_2_8_32_2","unstructured":"Russell\u2010Lodrique K.E.et al.2005.C. burnetiivirulence is isolate\u2010group specific in guinea pig aerosol\u2010challenge model.In4th International Conference on Rickettsiae and Rickettsial Diseases. Logrono Spain. Trama Impresores S.A.L."},{"key":"e_1_2_8_33_2","first-page":"45","article-title":"The biologic properties of Coxiella burnetii isolated from rodents collected in Utah","volume":"71","author":"Stoenner H.G.","year":"1960","journal-title":"Am. J. Hyg."},{"key":"e_1_2_8_34_2","unstructured":"Keim P. 2004. 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