{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T22:25:26Z","timestamp":1775773526065,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,9,5]],"date-time":"2006-09-05T00:00:00Z","timestamp":1157414400000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract:\u2002<\/jats:sc><\/jats:bold>  <jats:bold>Fetal cell\u2010free DNA (cf\u2010DNA) represents only a small fraction of the total cf\u2010DNA in maternal plasma. This feature has rendered it difficult to reliably distinguish fetal alleles which are not very disparate from maternal ones, such as those involving point mutations, by conventional polymerase chain reaction (PCR)\u2010based approaches. It has recently been shown that cell\u2010free fetal DNA molecules have a smaller size than comparable cf\u2010DNA molecules of maternal origin, and that this feature can be exploited for the selective enrichment of fetal DNA sequences, thereby permitting the detection of otherwise masked fetal genetic traits. By the use of this approach, we have shown that it is possible to detect fetal genetic loci for microsatellite markers, as well as point mutations involved in disorders such as achondroplasia and \u03b2\u2010thalassemia.<\/jats:bold> <\/jats:p>","DOI":"10.1196\/annals.1368.010","type":"journal-article","created":{"date-parts":[[2006,11,16]],"date-time":"2006-11-16T03:12:04Z","timestamp":1163646724000},"page":"81-87","source":"Crossref","is-referenced-by-count":15,"title":["Cell\u2010Free DNA in Maternal Plasma"],"prefix":"10.1111","volume":"1075","author":[{"given":"YING","family":"LI","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"WOLFGANG","family":"HOLZGREVE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"EDOARDO","family":"DI NARO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ANGELOANTONIO","family":"VITUCCI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SINUHE","family":"HAHN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,9,5]]},"reference":[{"key":"e_1_2_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)02174-0"},{"key":"e_1_2_3_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003081-200209000-00008"},{"key":"e_1_2_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0070-2153(04)61004-0"},{"key":"e_1_2_3_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2002.00165.x"},{"key":"e_1_2_3_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.AOG.0000103996.44503.F1"},{"key":"e_1_2_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)02767-7"},{"key":"e_1_2_3_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/301800"},{"key":"e_1_2_3_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/8.9.864"},{"key":"e_1_2_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2004.10.017"},{"key":"e_1_2_3_11_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2003.029835"},{"key":"e_1_2_3_12_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2003.024893"},{"key":"e_1_2_3_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1030"},{"key":"e_1_2_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1406"},{"key":"e_1_2_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/6.6.571"},{"key":"e_1_2_3_16_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.293.7.843"}],"container-title":["Annals of the New York Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1196%2Fannals.1368.010","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1196\/annals.1368.010","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T04:01:55Z","timestamp":1695614515000},"score":1,"resource":{"primary":{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/10.1196\/annals.1368.010"}},"subtitle":["Is It All a Question of Size?"],"short-title":[],"issued":{"date-parts":[[2006,9]]},"references-count":15,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2006,9]]}},"alternative-id":["10.1196\/annals.1368.010"],"URL":"https:\/\/doi.org\/10.1196\/annals.1368.010","archive":["Portico"],"relation":{},"ISSN":["0077-8923","1749-6632"],"issn-type":[{"value":"0077-8923","type":"print"},{"value":"1749-6632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,9]]}}}