{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:56:03Z","timestamp":1761058563041},"reference-count":12,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,9,5]],"date-time":"2006-09-05T00:00:00Z","timestamp":1157414400000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract:\u2002<\/jats:sc><\/jats:bold>  <jats:bold>In order to provide a noninvasive prenatal diagnosis of \u03b1<jats:sup>0<\/jats:sup>\u2010Thalassemia (Southeast Asian [SEA] deletion), we have developed a real\u2010time quantitative semi\u2010nested polymerase chain reaction (PCR) method for identifying the fetal \u03b1<jats:sup>0<\/jats:sup>\u2010Thalassemia in maternal plasma. Analysis was performed using DNA extracted from 200 \u03bcL plasma from 13 pregnant women during 8\u201320 weeks of gestation who carried fetuses with normal (2), \u03b1<jats:sup>0<\/jats:sup>\u2010Thalassemia carrier (8), Hb H disease (1), and homozygous \u03b1<jats:sup>0<\/jats:sup>\u2010Thalassemia (Hb Bart's hydrops fetalis (2). The \u03b1<jats:sup>0<\/jats:sup>\u2010Thalassemia was detected using a two\u2010step PCR. Plasma DNA was amplified conventionally using \u03b1<jats:sup>0<\/jats:sup>\u2010Thalassemia\u2010specific primers and a portion of the first PCR product was subjected to a semi\u2010nested real\u2010time q\u2010PCR using the SYBR green I chemistry for fluorescence detection. Calibration curve for \u03b1<jats:sup>0<\/jats:sup>\u2010Thalassemia quantification was prepared by assaying serial dilution of genomic DNA of an \u03b1<jats:sup>0<\/jats:sup>\u2010Thalassemia carrier. Differences in the C<jats:sub>T<\/jats:sub> (threshold cycle) values and calculated concentrations of amplified DNA among normal fetus, \u03b1<jats:sup>0<\/jats:sup>\u2010Thalassemia carrier, Hb H disease, and homozygous \u03b1<jats:sup>0<\/jats:sup>\u2010Thalassemia were clearly observed, which could help in prenatal prediction of the fetal genotype. This noninvasive prenatal detection of \u03b1<jats:sup>0<\/jats:sup>\u2010Thalassemia in maternal plasma should enhance prenatal diagnostic options for this common genetic disorder in routine DNA diagnostic setting.<\/jats:bold> <\/jats:p>","DOI":"10.1196\/annals.1368.013","type":"journal-article","created":{"date-parts":[[2006,11,16]],"date-time":"2006-11-16T03:12:04Z","timestamp":1163646724000},"page":"103-107","source":"Crossref","is-referenced-by-count":41,"title":["Development and Application of a Real\u2010Time Quantitative PCR for Prenatal Detection of Fetal \u03b1<sup>0<\/sup>\u2010Thalassemia from Maternal Plasma"],"prefix":"10.1111","volume":"1075","author":[{"given":"WARUNEE","family":"TUNGWIWAT","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SUPAN","family":"FUCHAROEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GOONNAPA","family":"FUCHAROEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"THAWALWONG","family":"RATANASIRI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KANOKWAN","family":"SANCHAISURIYA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,9,5]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.3109\/03630268709036587"},{"key":"e_1_2_5_3_2","doi-asserted-by":"crossref","first-page":"2213","DOI":"10.1182\/blood.V91.7.2213","article-title":"Hydrops fetalis caused by \u03b10\u2010Thalassemia: an emerging health care problem","volume":"91","author":"Chui D.H.K.","year":"1998","journal-title":"Blood"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1159\/000064746"},{"key":"e_1_2_5_5_2","first-page":"364","article-title":"A simplified screening strategy for thalassaemia and haemoglobin E in rural communities in Southeast Asia","volume":"82","author":"Fucharoen G.","year":"2004","journal-title":"Bull. 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