{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T01:47:50Z","timestamp":1790905670315,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,11,17]],"date-time":"2006-11-17T00:00:00Z","timestamp":1163721600000},"content-version":"vor","delay-in-days":47,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract:\u2002<\/jats:sc><\/jats:bold>  <jats:bold>Bovine babesiosis, caused by <jats:italic>Babesia bovis<\/jats:italic>, <jats:italic>B. bigemina<\/jats:italic>, and <jats:italic>B. divergens<\/jats:italic>, is a significant impediment to livestock production in countries with tropical\/subtropical and temperate climates. Previous studies conducted on the immunoprophylaxis against the disease and diagnosis of these parasites has demonstrated the presence of similar antigens. The objective of this article was to identify and partially characterize antigens conserved among these three species. Immunochemical analysis using sera from cattle immunized individually with antigens from these three <jats:italic>Babesia<\/jats:italic> species revealed a number of antigens recognized by heterologous antisera. Cross\u2010reactions were more evident in sera from cattle immunized with <jats:italic>B. bovis<\/jats:italic>\/<jats:italic>B. bigemina<\/jats:italic> which recognized several antigens (15 kDa to &gt;200 kDa) in <jats:italic>B. divergens<\/jats:italic>. Immunoscreening of a <jats:italic>B. divergens<\/jats:italic> cDNA library with bovine serum to <jats:italic>B. bigemina<\/jats:italic> allowed the isolation of five clones and DNA sequencing of plasmid BdJF5 showed a 680 bp cDNA insert. Basic Local Alignment Search Tool (BLAST) analysis of the predicted amino acid sequence revealed 47% identity with a protein identified as \u03b1NAC.<\/jats:bold>\n          <jats:bold>Serum from mice immunized with a recombinant Glutathione S\u2010Transferase\u2010BdJF5 fusion protein immunoprecipitated a 20 kDa <jats:italic>B. bovis<\/jats:italic> antigen. However, 30 kDa and 18 kDa antigens were immunoprecipitated from <jats:italic>B. divergens<\/jats:italic> and immunoblotting analysis revealed the recognition of a 35 kDa <jats:italic>B. bigemina<\/jats:italic> antigen. An indirect fluorescence antibody assay on merozoites showed strong reaction with <jats:italic>B. divergens<\/jats:italic> and weak recognition of <jats:italic>B. bovis<\/jats:italic> and <jats:italic>B. bigemina<\/jats:italic>. Despite the existent antigenic polymorphism among the <jats:italic>Babesia<\/jats:italic> spp., these results demonstrated that common antigens occur between European <jats:italic>B. divergens<\/jats:italic> and Mexican <jats:italic>B. bovis\/B. bigemina<\/jats:italic>.<\/jats:bold> <\/jats:p>","DOI":"10.1196\/annals.1373.057","type":"journal-article","created":{"date-parts":[[2006,11,30]],"date-time":"2006-11-30T00:48:38Z","timestamp":1164847718000},"page":"382-396","source":"Crossref","is-referenced-by-count":5,"title":["Identification of Common Antigens in <i>Babesia bovis<\/i>, <i>B. bigemina<\/i>, and <i>B. divergens<\/i>"],"prefix":"10.1111","volume":"1081","author":[{"given":"JULIO V.","family":"FIGUEROA","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ERIC","family":"PRECIGOUT","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"BERNARD","family":"CARCY","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ANDR\u00c9","family":"GORENFLOT","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,11,17]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"1","volume-title":"Babesiosis","author":"McCosker P.J.","year":"1981"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1980.tb04228.x"},{"key":"e_1_2_6_4_2","first-page":"519","volume-title":"Pr\u00e9cis De Parasitologie V\u00e9terinaire Tropicale","author":"Morel P.C.","year":"2000"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182004005190"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.16.4.622-636.2003"},{"key":"e_1_2_6_7_2","first-page":"1","volume-title":"Ticks and Tick\u2010Borne Diseases: Proceedings of the 56th Annual Conference of the Australian Veterinary Association","author":"Uilenberg G.","year":"1980"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-009-6042-8_7"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4017(94)03112-A"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5288(18)34012-8"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-003-1060-x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(98)00095-8"},{"key":"e_1_2_6_13_2","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1128\/iai.56.6.1658-1660.1988","article-title":"Identification of Babesia bigemina and Babesia bovis merozoite proteins with isolate\u2010 and species\u2010common epitopes recognized by antibodies in bovine immune sera","volume":"56","author":"McElwain T.F.","year":"1988","journal-title":"Infect. Immun"},{"key":"e_1_2_6_14_2","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1128\/iai.55.2.364-368.1987","article-title":"Protection of Babesia bigemina\u2010immune animals against subsequent challenge with virulent Babesia bovis","volume":"55","author":"Wright I.G.","year":"1987","journal-title":"Infect. Immun"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000052835"},{"key":"e_1_2_6_16_2","first-page":"416","article-title":"In vitro cultivation of Babesia bigemina","volume":"46","author":"Vega C.A.","year":"1985","journal-title":"Am. J. Vet. Res"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00934377"},{"key":"e_1_2_6_18_2","first-page":"13","article-title":"Insuficiente inmunidad cruzada en bovinos por Babesia bigemina y\/o Babesia bovis derivadas de cultivo in vitro","volume":"37","author":"Vega C.A.","year":"1999","journal-title":"Tec. Pecu. Mex"},{"key":"e_1_2_6_19_2","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1128\/iai.61.2.734-741.1993","article-title":"Cellular and humoral immune responses induced in cattle by vaccination with Babesia divergens culture\u2010derived exoantigens correlate with protection","volume":"61","author":"Valentin A.","year":"1993","journal-title":"Infect. Immun"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1307.017"},{"key":"e_1_2_6_21_2","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli U.K.","year":"1972","journal-title":"Nature"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182002002160"},{"key":"e_1_2_6_24_2","volume-title":"Molecular Cloning: a Laboratory Manual","author":"Sambrock J.","year":"2001"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5463"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki069"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.278202"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/370434a0"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M006368200"}],"container-title":["Annals of the New York Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1196%2Fannals.1373.057","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1196\/annals.1373.057","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T06:57:04Z","timestamp":1695625024000},"score":1,"resource":{"primary":{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/10.1196\/annals.1373.057"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10]]},"references-count":29,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2006,10]]}},"alternative-id":["10.1196\/annals.1373.057"],"URL":"https:\/\/doi.org\/10.1196\/annals.1373.057","archive":["Portico"],"relation":{},"ISSN":["0077-8923","1749-6632"],"issn-type":[{"value":"0077-8923","type":"print"},{"value":"1749-6632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,10]]}}}