{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T09:08:32Z","timestamp":1761988112669},"reference-count":12,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,11,17]],"date-time":"2006-11-17T00:00:00Z","timestamp":1163721600000},"content-version":"vor","delay-in-days":47,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract:\u2002<\/jats:sc><\/jats:bold>  <jats:bold>A comparative assessment of the virulence of <jats:italic>Babesia bovis<\/jats:italic> clones that adhere or not to bovine brain endothelial cells was done using two clones of <jats:italic>B. bovis<\/jats:italic>: (1) a clone phenotypically characterized as virulent (2F8) and (2) a clone of reduced virulence (RAD). Of these subpopulations, we selected those that had adhesive characteristics (a) or nonadhesive characteristics (na) in cultured endothelial cells. Twenty Holstein cattle, 12 months of age or older, were used in this study, and these cattle were randomly assigned to five groups of four animals each. The clones and their respective subpopulations were inoculated via intramuscular injection at a 0.5 \u00d7 10<jats:sup>7<\/jats:sup> infected erythrocyte dosage. Group A was inoculated with aRAD, group B with naRAD, group C with a2F8, group D with na2F8, and group E remained as a control. All inoculated animals showed a decrease in the packed cell volume (PCV), with group D showing the largest decrease (39.53%) and longest time (7 days) with rectal temperature above 39.5\u00b0C. <jats:italic>Babesia<\/jats:italic> was observed in stained blood smears from only six cattle. While the four parasite subpopulations were pathogenic, significant differences were not noted among them, despite that the subpopulations considered to be virulent caused the greatest reduction in PCV per individual.<\/jats:bold> <\/jats:p>","DOI":"10.1196\/annals.1373.058","type":"journal-article","created":{"date-parts":[[2006,11,30]],"date-time":"2006-11-30T00:48:38Z","timestamp":1164847718000},"page":"397-404","source":"Crossref","is-referenced-by-count":7,"title":["Evaluation of Cattle Inoculated with <i>Babesia bovis<\/i> Clones Adhesive <i>In Vitro<\/i> to Bovine Brain Endothelial Cells"],"prefix":"10.1111","volume":"1081","author":[{"given":"GERMINAL J.","family":"CANTO","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JULIO V.","family":"FIGUEROA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JUAN A.","family":"RAMOS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"EDMUNDO E.","family":"ROJAS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DAVID","family":"GARCIA\u2010TAPIA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"ANTONIO ALVAREZ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DAVID R.","family":"ALLRED","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"CHARLES A.","family":"CARSON","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,11,17]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"1","volume-title":"Babesiosis","author":"McCosker P.J.","year":"1981"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(88)90161-5"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050691"},{"key":"e_1_2_6_5_2","doi-asserted-by":"crossref","first-page":"3921","DOI":"10.1128\/IAI.67.8.3921-3928.1999","article-title":"Cytoadherence of Babesia bovis\u2010infected erythrocytes to bovine brain capillary endothelial cells provides an in vitro model for sequestration","volume":"67","author":"O'Connor R.M.","year":"1999","journal-title":"Infect. 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