{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T06:07:28Z","timestamp":1695708448654},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,11,17]],"date-time":"2006-11-17T00:00:00Z","timestamp":1163721600000},"content-version":"vor","delay-in-days":47,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract:\u2002<\/jats:sc><\/jats:bold>  <jats:bold>A new pathogenic <jats:italic>Theileria<\/jats:italic> species transmitted by <jats:italic>Haemaphysalis qinghaiensis<\/jats:italic> was identified in the Northwestern part of China and was shown to be highly pathogenic for small ruminants. The present article aimed at identifying merozoite antigens that might be suitable for developing diagnostic methods and designing a potential vaccine. Absence of other theilerial or babesial infections was confirmed by reverse line blot in all antigen samples used. Extensive Western blot analyses using serum from infected and noninfected animals led to the identification of four potential merozoite immunoreactive proteins at different molecular weights. Further protein characterization using peptide mass mapping by matrix\u2010assisted laser desorption\/ionization (MALDI) followed by database searching resulted in two significant hits that identified two proteins of parasite origin, one homologous to a possible MO25\u2010family protein from <jats:italic>Cryptosporidium parvum<\/jats:italic> and the other with an HSP70 from <jats:italic>Theileria annulata.<\/jats:italic> Another protein was also identified as a parasite protein but without significant homology. Immunization of rabbits with selected proteins produced antisera that reacted specifically on Western blots with merozoite antigens of the corresponding sizes. This article represents the first identification and characterization of potential antigenic proteins of <jats:italic>Theileria<\/jats:italic> sp. (China) for veterinary purposes.<\/jats:bold> <\/jats:p>","DOI":"10.1196\/annals.1373.063","type":"journal-article","created":{"date-parts":[[2006,11,30]],"date-time":"2006-11-30T00:48:38Z","timestamp":1164847718000},"page":"443-452","source":"Crossref","is-referenced-by-count":1,"title":["Identification and Characterization of Merozoite Antigens of a <i>Theileria<\/i> Species Highly Pathogenic for Small Ruminants in China"],"prefix":"10.1111","volume":"1081","author":[{"given":"JOANA","family":"MIRANDA","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ELISABETE","family":"NASCIMENTO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HELDER","family":"CRUZ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YIN","family":"HONG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ANA","family":"COELHO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JABBAR S.","family":"AHMED","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ABEL","family":"OLIVA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,11,17]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(08)60285-7"},{"key":"e_1_2_6_3_2","first-page":"99","article-title":"Tick\u2010borne diseases of sheep and goats caused by Babesia, Theileria and Anaplasma spp","volume":"39","author":"Friedhoff K.T.","year":"1997","journal-title":"Parasitologia"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02632907"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050680"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2000.tb05300.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-001-0563-6"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-001-0565-4"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-002-0611-x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(02)00166-8"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-003-0979-2"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02236144"},{"key":"e_1_2_6_13_2","first-page":"119","article-title":"Further studies on Haematoxenus separatus (Sporozoa, Theileriidae) of sheep in Tanzania","volume":"29","author":"Uilenberg G.","year":"1976","journal-title":"Rev. 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