{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:00:04Z","timestamp":1791288004592,"version":"4.3.0"},"reference-count":4,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,10,31]],"date-time":"2006-10-31T00:00:00Z","timestamp":1162252800000},"content-version":"vor","delay-in-days":30,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract:\u2002<\/jats:sc><\/jats:bold>  <jats:bold>We evaluated real\u2010time PCR assays for the detection of <jats:italic>C. burnetii<\/jats:italic> which targets sequences that are present either in one (<jats:italic>icd<\/jats:italic>) or in several copies (transposase of <jats:italic>IS1111a<\/jats:italic>) on the chromosome. The assays are highly sensitive, with reproducible detection limits of approximately 10 copies per reaction, at least 100 times more sensitive than capture ELISA, when performed on infected placenta material and specific for <jats:italic>C. burnetii<\/jats:italic>. The numbers of <jats:italic>IS1111<\/jats:italic> elements in the genomes of 75 <jats:italic>C. burnetii<\/jats:italic> isolates were quantified by real\u2010time PCR and proved to be highly variable.<\/jats:bold> <\/jats:p>","DOI":"10.1196\/annals.1374.111","type":"journal-article","created":{"date-parts":[[2006,11,18]],"date-time":"2006-11-18T02:13:56Z","timestamp":1163816036000},"page":"563-565","source":"Crossref","is-referenced-by-count":26,"title":["Evaluation of a Real\u2010Time PCR Assay to Detect <i>Coxiella burnetii<\/i>"],"prefix":"10.1111","volume":"1078","author":[{"given":"SILKE R.","family":"KLEE","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HEINZ","family":"ELLERBROK","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JUDITH","family":"TYCZKA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"TATJANA","family":"FRANZ","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"BERND","family":"APPEL","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,10,31]]},"reference":[{"key":"e_1_2_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.17.5540-5548.1992"},{"key":"e_1_2_3_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0931379100"},{"key":"e_1_2_3_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1999.tb08803.x"},{"key":"e_1_2_3_5_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268897008510"}],"container-title":["Annals of the New York Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1196%2Fannals.1374.111","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1196\/annals.1374.111","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T19:05:35Z","timestamp":1695668735000},"score":1,"resource":{"primary":{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/10.1196\/annals.1374.111"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10]]},"references-count":4,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2006,10]]}},"alternative-id":["10.1196\/annals.1374.111"],"URL":"https:\/\/doi.org\/10.1196\/annals.1374.111","archive":["Portico"],"relation":{},"ISSN":["0077-8923","1749-6632"],"issn-type":[{"value":"0077-8923","type":"print"},{"value":"1749-6632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,10]]}}}