{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T02:48:40Z","timestamp":1792723720872,"version":"4.5.0"},"reference-count":3,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,10,31]],"date-time":"2006-10-31T00:00:00Z","timestamp":1162252800000},"content-version":"vor","delay-in-days":30,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract:\u2002<\/jats:sc><\/jats:bold>  <jats:bold>Diagnosis of Q fever has largely been dependent upon serology, which may lead to delayed diagnosis as seroconversion can take weeks to develop. During a recent Q fever outbreak (27 patients) in rural South Australia, we compared the diagnostic rate of serology with two separate real\u2010time PCRs, the 27kDa outer membrane protein and the insertion sequence. PCR was positive (on either or both PCR assays) in sera of 67% of the patients. Median time required for making serological diagnosis was 17 days, compared with 4 days by PCR. Q fever PCR is an effective tool in the diagnosis of acute Q fever infection.<\/jats:bold> <\/jats:p>","DOI":"10.1196\/annals.1374.112","type":"journal-article","created":{"date-parts":[[2006,11,18]],"date-time":"2006-11-18T02:13:56Z","timestamp":1163816036000},"page":"566-569","source":"Crossref","is-referenced-by-count":39,"title":["Diagnosis of Acute Q Fever by PCR on Sera during a Recent Outbreak in Rural South Australia"],"prefix":"10.1111","volume":"1078","author":[{"given":"M.","family":"TURRA","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"CHANG","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"WHYBROW","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"HIGGINS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"QIAO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,10,31]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-19-3-281"},{"key":"e_1_2_5_3_2","first-page":"338","article-title":"Early recognition of a 27 kDa membrane protein (MP27) in Coxiella burnetii infected and vaccinated guinea pigs","volume":"35","author":"Schmeer N.","year":"1988","journal-title":"Zentralbl. Veterinarmed. B."},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0931379100"}],"container-title":["Annals of the New York Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1196%2Fannals.1374.112","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1196\/annals.1374.112","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T19:05:37Z","timestamp":1695668737000},"score":1,"resource":{"primary":{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/10.1196\/annals.1374.112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10]]},"references-count":3,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2006,10]]}},"alternative-id":["10.1196\/annals.1374.112"],"URL":"https:\/\/doi.org\/10.1196\/annals.1374.112","archive":["Portico"],"relation":{},"ISSN":["0077-8923","1749-6632"],"issn-type":[{"value":"0077-8923","type":"print"},{"value":"1749-6632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,10]]}}}