{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T20:58:44Z","timestamp":1761512324576},"reference-count":10,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,10,31]],"date-time":"2006-10-31T00:00:00Z","timestamp":1162252800000},"content-version":"vor","delay-in-days":30,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract:\u2002<\/jats:sc><\/jats:bold>  <jats:bold>Two strategies to improve the efficacy of the shell\u2010vial method for <jats:italic>Rickettsia<\/jats:italic> were analyzed. Blood samples from 59 patients with Mediterranean spotted fever (MSF) were examined using the shell\u2010vial technique. (i) DNA from positive lenses was obtained when they were contaminated. (ii) Blood sample from one patient was cultured in 17 shell\u2010vials. <jats:italic>R. conorii<\/jats:italic> was identified in four cases by polymerase chain reaction (PCR)\u2010RFLP<\/jats:bold>. <jats:bold>Three of these were obtained from cells adherent to lenses and the fourth one by using total patient blood sample. <jats:italic>Rickettsia<\/jats:italic> isolation using all blood samples as well as DNA from shell\u2010vial lenses could be useful in the study of rickettsial infections.<\/jats:bold><\/jats:p>","DOI":"10.1196\/annals.1374.115","type":"journal-article","created":{"date-parts":[[2006,11,18]],"date-time":"2006-11-18T02:13:56Z","timestamp":1163816036000},"page":"578-581","source":"Crossref","is-referenced-by-count":6,"title":["Ten Years' Experience of Isolation of <i>Rickettsia<\/i> spp. from Blood Samples Using the Shell\u2010Vial Cell Culture Assay"],"prefix":"10.1111","volume":"1078","author":[{"given":"MARIELA","family":"QUESADA","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ISABEL","family":"SANFELIU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"NEUS","family":"CARDE\u00d1OSA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"FERRAN","family":"SEGURA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,10,31]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"crossref","first-page":"142","DOI":"10.4269\/ajtmh.2000.62.142","article-title":"Isolation and identification of two spotted fever group rickettsial strains from patients in Catalonia, Spain","volume":"62","author":"Carde\u00f1osa N.","year":"2000","journal-title":"Am. 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