{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T14:06:55Z","timestamp":1768399615605,"version":"3.49.0"},"reference-count":8,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,10,31]],"date-time":"2006-10-31T00:00:00Z","timestamp":1162252800000},"content-version":"vor","delay-in-days":30,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract:\u2002<\/jats:sc><\/jats:bold>  <jats:bold>Using the indirect enzyme\u2010linked immunosorbent assay (ELISA) technique, we compared the immune response of specific antibodies (IgM and IgG) in serum samples of scrub typhus\u2010infected and non\u2010infected mice against <jats:italic>Orientia sutsugamushi<\/jats:italic> (a causative agent of scrub typhus). Two different age groups (4\u2010week\u2010old and 10\u2010week\u2010old) of ICR laboratory mice were infected with <jats:italic>O. tsutsugamushi<\/jats:italic> by the animal passage procedure. Serum samples were detected for scrub typhus\u2010specific antibodies using ELISA technique. Results from determining the presence of IgM and IgG in the serum samples obtained from scrub typhus\u2010infected mice showed that the IgG was first detected on day\u201013 after the initial infection in both the 4\u2010week\u2010old and 10\u2010week\u2010old mice. The IgG titer levels of both groups were not significantly different. Although the presence of IgM in the in serum samples obtained from scrub typhus\u2010infected mice was first detected on day 13 in the 4\u2010week\u2010old mice and on day\u201012 in the 10\u2010week\u2010old mice, the IgM titer in the 4\u2010week\u2010old mice was slightly lower than in the 10\u2010week\u2010old mice. Clinical observations of the scrub typhus\u2010infected mice showed that the older mice become ill on day 9 whereas the younger mice exhibited the symptoms on day 12. Considering the earlier detection and slightly higher level of specific IgM antibody, it could be interpreted that the older mice may appear to have responded against <jats:italic>O. tsutsugamushi<\/jats:italic> faster than the younger mice.<\/jats:bold> <\/jats:p>","DOI":"10.1196\/annals.1374.121","type":"journal-article","created":{"date-parts":[[2006,11,18]],"date-time":"2006-11-18T02:13:56Z","timestamp":1163816036000},"page":"607-612","source":"Crossref","is-referenced-by-count":5,"title":["Comparison of Immune Response against <i>Orientia tsutsugamushi<\/i>, a Causative Agent of Scrub Typhus, in 4\u2010Week\u2010Old and 10\u2010Week\u2010Old Scrub Typhus\u2010Infected Laboratory Mice Using Enzyme\u2010Linked Immunosorbent Assay Technique"],"prefix":"10.1111","volume":"1078","author":[{"given":"KRIANGKRAI","family":"LERDTHUSNEE","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SUPAK","family":"JENKITKASEMWONG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SUCHEERA","family":"INSUAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"WARISA","family":"LEEPITAKRAT","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"TAWEESAK","family":"MONKANNA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"NITTAYA","family":"KHLAIMANEE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"WEERAYUT","family":"CHAREONSONGSERMKIJ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SURACHAI","family":"LEEPITAKRAT","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KWANTA","family":"CHAYAPHUM","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JAMES. W.","family":"JONES","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,10,31]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1603\/0022-2585-39.3.521"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2003.tb07333.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2003.69.519"},{"key":"e_1_2_6_5_2","first-page":"38","article-title":"ELISA for antibodies against scrub typhus","volume":"9","author":"Dasch G.A.","year":"1979","journal-title":"J. Clin. 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