{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T07:31:57Z","timestamp":1773300717509,"version":"3.50.1"},"reference-count":11,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,1,26]],"date-time":"2007-01-26T00:00:00Z","timestamp":1169769600000},"content-version":"vor","delay-in-days":56,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract:\u2002<\/jats:sc><\/jats:bold>  <jats:bold>Apoptosis linked to oxidative stress has been implicated in pancreatitis. NADPH oxidase has been considered as a major source of reactive oxygen species (ROS) during inflammation and apoptosis in pancreatic acinar cells. Recently we demonstrated that NADPH oxidase subunits Nox1, p27<jats:sup>phox<\/jats:sup>, p47<jats:sup>phox<\/jats:sup>, and p67<jats:sup>phox<\/jats:sup> are constitutively expressed in pancreatic acinar cells and may contribute to apoptosis in pancreatic acinar AR42J cells stimulated with cerulein. The present study aims to investigate the apoptotic mechanism of pancreatic acinar cells stimulated with cerulein by determining whether cerulein induces apoptosis\u2010inducing factor (AIF) expression and whether cerulein\u2010induced expression of AIF is inhibited by transfection with antisense oligonucleotide (AS ODN) of p47<jats:sup>phox<\/jats:sup> or p67<jats:sup>phox<\/jats:sup> or treatment with a Ca<jats:sup>2+<\/jats:sup> chelator BAPTA\u2010AM. As a result, cerulein induced the expression of apoptotic gene AIF. Transfection with AS ODN of p47<jats:sup>phox<\/jats:sup> or p67<jats:sup>phox<\/jats:sup> or treatment with BAPTA\u2010AM inhibited cerulein\u2010induced AIF expression in pancreatic acinar AR42J cells. These results demonstrate that NADPH oxidase and calcium have a role in cerulein\u2010induced apoptosis in pancreatic acinar AR42J cells by inducing the expression of AIF. In conclusion, the increase in intracellular Ca<jats:sup>2+<\/jats:sup> and NADPH oxidase activity may be the upstream event of apoptotic gene (AIF) expression, which contributes to cerulein\u2010induced apoptosis in pancreatic acinar AR42 cells.<\/jats:bold> <\/jats:p>","DOI":"10.1196\/annals.1378.031","type":"journal-article","created":{"date-parts":[[2007,3,23]],"date-time":"2007-03-23T19:12:40Z","timestamp":1174677160000},"page":"292-297","source":"Crossref","is-referenced-by-count":29,"title":["Role of NADPH Oxidase and Calcium in Cerulein\u2010Induced Apoptosis"],"prefix":"10.1111","volume":"1090","author":[{"given":"JI HOON","family":"YU","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KYUNG HWAN","family":"KIM","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HYEYOUNG","family":"KIM","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,1,26]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"crossref","first-page":"1306S","DOI":"10.1093\/ajcn\/62.6.1306S","article-title":"Oxidative stress in acute and chronic pancreatitis","volume":"62","author":"Schenberg M.H.","year":"1995","journal-title":"Am. J. Clin. Nutr."},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.1800771119"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.269.5.C1295"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1076194"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207517"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2005.04.019"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI3437"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2005.02.004"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.24.13126"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(96)70077-X"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119714"}],"container-title":["Annals of the New York Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1196%2Fannals.1378.031","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1196\/annals.1378.031","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T13:44:27Z","timestamp":1695649467000},"score":1,"resource":{"primary":{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/10.1196\/annals.1378.031"}},"subtitle":["Involvement of Apoptosis\u2010Inducing Factor"],"short-title":[],"issued":{"date-parts":[[2006,12]]},"references-count":11,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2006,12]]}},"alternative-id":["10.1196\/annals.1378.031"],"URL":"https:\/\/doi.org\/10.1196\/annals.1378.031","archive":["Portico"],"relation":{},"ISSN":["0077-8923","1749-6632"],"issn-type":[{"value":"0077-8923","type":"print"},{"value":"1749-6632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,12]]}}}