{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:23:52Z","timestamp":1791149032376,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,1,26]],"date-time":"2007-01-26T00:00:00Z","timestamp":1169769600000},"content-version":"vor","delay-in-days":56,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract:\u2002<\/jats:sc><\/jats:bold>  <jats:bold>Purified <jats:italic>Salmonella typhimurium<\/jats:italic> DNA was incubated for 1h at 37\u00b0C with various concentrations (10\u2013100 \u03bcM) of transition metal ions (Fe<jats:sup>2+<\/jats:sup>, Fe<jats:sup>3+<\/jats:sup>, Cu<jats:sup>2+<\/jats:sup>, Ni<jats:sup>2+<\/jats:sup>, Cd<jats:sup>2+<\/jats:sup>), with various concentrations (0.1\u2013100 mM) of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, and with various concentrations of each transition metal ion in the presence of various concentrations of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>. Damage to DNA was assessed by electrophoresis of the reaction mixtures in 1% agarose gel. Breakage of the DNA strands would produce a series of DNA fragments resulting in a smear in the gel, while intact DNA produced a single band. Results showed that no damage to the DNA was detectable after incubation with either H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> alone or either of the metal ions alone. However, all of the metal ions investigated triggered DNA breakage in the presence of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>. The extent of breakage depended on the metal ion and on its concentration, as well as on the H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> concentration. Addition of either EDTA or catalase to the reaction mixture completely inhibited the DNA degradation, confirming the involvement of both the metal ion and the H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> in the breakage of DNA strands. Production of the hydroxyl radical when H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> and a metal ion were both present in the reaction mixture was evidenced by the thiobarbituric acid method. The most extensive damage was caused by Cu<jats:sup>2+<\/jats:sup> followed, in decreasing order, by Fe<jats:sup>2+<\/jats:sup>, Fe<jats:sup>3+<\/jats:sup>, Ni<jats:sup>2+<\/jats:sup>, and Cd<jats:sup>2+<\/jats:sup>.<\/jats:bold> <\/jats:p>","DOI":"10.1196\/annals.1378.054","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T18:27:35Z","timestamp":1173205655000},"page":"52-64","source":"Crossref","is-referenced-by-count":8,"title":["DNA Strand Breaks by Metal\u2010Induced Oxygen Radicals in Purified <i>Salmonella typhimurium<\/i> DNA"],"prefix":"10.1111","volume":"1091","author":[{"given":"EZZATOLLAH","family":"KEYHANI","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FATEMEH","family":"ABDI\u2010OSKOUEI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FARNOOSH","family":"ATTAR","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JACQUELINE","family":"KEYHANI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,1,26]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.49.070180.002041"},{"key":"e_1_2_6_3_2","volume-title":"Nutritional Biochemistry and Metabolism","author":"Linder M.C.","year":"1991"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1974.tb06788.x"},{"key":"e_1_2_6_5_2","first-page":"72","article-title":"Screening for hemochromatosis phenotype versus genotype","volume":"35","author":"Edwards C.Q.","year":"1998","journal-title":"Semin. 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