{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T04:43:25Z","timestamp":1696826605083},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,1,26]],"date-time":"2007-01-26T00:00:00Z","timestamp":1169769600000},"content-version":"vor","delay-in-days":56,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract:\u2002<\/jats:sc><\/jats:bold>  <jats:bold>Previous studies reported that constitutive androstane receptor (CAR) does not transactivate phenobarbital responsive unit (PBRU)2C1luciferase reporter gene in COS cells in which endogenous <jats:italic>CYP2B1<\/jats:italic> gene is not induced with PB. In order to understand molecular mechanism(s) whereby PBRU is transactivated, this article determined if the use of strong thymidine kinase (TK) promotor rather than the minimal <jats:italic>CYP2C1<\/jats:italic> promotor, and hepatocyte nuclear factor\u20104 (HNF\u20104) can affect CAR\u2010mediated transactivation of PBRU in the monkey kidney epithelial\u2010derived COS\u20107 cells. To examine CAR\u2010mediated transactivation, cultured COS\u20107 cells were transfected with CAR expression plasmid, pEGFP\u2010mCAR1, and confirmed for high level of the protein expression. In COS\u20107 cells, TK promotor induced CAR\u2010mediated PBRU transactivation in a dose\u2010dependent manner. Whereas expression of HNF\u20104 slightly promoted PBRU transactivation with low amount of CAR transfected, it repressed PBRU transactivation in a dose\u2010dependent manner with high amount of CAR. Consistent with the previous reports in Hep G2 cells, CAR transactivated PBRU2C1luciferase in a dose\u2010dependent manner and this CAR\u2010mediated transactivation required functional NR\u20101 and NF\u20101 sites. However, HNF\u20104 did not affect CAR\u2010mediated PBRU transactivation in Hep G2 cells. These results suggest that proximal promotor and a <jats:italic>trans<\/jats:italic>\u2010acting factor, HNF\u20104, can affect CAR\u2010mediated transactivation of PBRU in COS\u20107 cells.<\/jats:bold> <\/jats:p>","DOI":"10.1196\/annals.1378.072","type":"journal-article","created":{"date-parts":[[2007,3,6]],"date-time":"2007-03-06T18:27:35Z","timestamp":1173205655000},"page":"258-269","source":"Crossref","is-referenced-by-count":0,"title":["Effects of TK Promotor and Hepatocyte Nuclear Factor\u20104 in CAR\u2010Mediated Transcriptional Activity of Phenobarbital Responsive Unit of <i>CYP2B<\/i> Gene in Monkey Kidney Epithelial\u2010Derived Cell Line COS\u20107"],"prefix":"10.1111","volume":"1091","author":[{"given":"GYESIK","family":"MIN","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,1,26]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00008571-199602000-00002"},{"key":"e_1_2_6_3_2","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1016\/S0021-9258(18)70924-1","article-title":"The enzymatic deamination of amphetamine (benzedrine)","volume":"214","author":"Axelrod J.","year":"1955","journal-title":"J. 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