{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T08:31:13Z","timestamp":1792225873933,"version":"4.4.0"},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,7,16]],"date-time":"2007-07-16T00:00:00Z","timestamp":1184544000000},"content-version":"vor","delay-in-days":45,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract:\u2002<\/jats:sc><\/jats:bold>  <jats:bold>The isolation of mesenchymal stem cells (MSC) from primary tissue is hampered by the limited selectivity of available markers. So far, CD271 is one of the most specific markers for bone marrow (BM)\u2010derived MSC. In search of additional markers, monoclonal antibodies (mAbs) with specificity for immature cells were screened by flow cytometry for their specific reactivity with the rare CD271<jats:sup>+<\/jats:sup> population. The recognized CD271<jats:sup>+<\/jats:sup> populations were fractionated by fluorescence\u2010activated cell sorting and the clonogenic capacity of the sorted cells was analyzed for their ability to give rise to CFU\u2010F. The results showed that only the CD271<jats:sup>bright<\/jats:sup> but not the CD271<jats:sup>dim<\/jats:sup> population contained CFU\u2010F. Two\u2010color flow cytometry analysis revealed that only the CD271<jats:sup>bright<\/jats:sup> population was positive for the established MSC markers CD10, CD13, CD73, and CD105. In addition, a variety of mAbs specific for novel and partially unknown antigens selectively recognized the CD271<jats:sup>bright<\/jats:sup> population but no other BM cells. The new MSC\u2010specific molecules included the platelet\u2010derived growth factor receptor\u2010\u03b2 (CD140b), HER\u20102\/erbB2 (CD340), frizzled\u20109 (CD349), the recently described W8B2 antigen, as well as cell\u2010surface antigens defined by the antibodies W1C3, W3D5, W4A5, W5C4, W5C5, W7C6, 9A3, 58B1, F9\u20103C2F1, and HEK\u20103D6. In conclusion, the described markers are suitable for the prospective isolation of highly purified BM\u2010MSC. These MSC may be used as an improved starting population for transplantation in diseases like osteogenesis imperfecta, cartilage repair, and myocardial infarction.<\/jats:bold> <\/jats:p>","DOI":"10.1196\/annals.1392.000","type":"journal-article","created":{"date-parts":[[2007,7,19]],"date-time":"2007-07-19T19:28:24Z","timestamp":1184873304000},"page":"262-271","source":"Crossref","is-referenced-by-count":372,"title":["Novel Markers for the Prospective Isolation of Human MSC"],"prefix":"10.1111","volume":"1106","author":[{"given":"HANS\u2010J\u00d6RG","family":"B\u00dcHRING","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"VENKATA LOKESH","family":"BATTULA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SABRINA","family":"TREML","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"BERNHARD","family":"SCHEWE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LOTHAR","family":"KANZ","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"WICHARD","family":"VOGEL","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,7,16]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-472X(00)00482-3"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2000.01986.x"},{"key":"e_1_2_6_4_2","first-page":"267","article-title":"Fibroblast precursors in normal and irradiated mouse hematopoietic organs","volume":"4","author":"Friedenstein A.J.","year":"1976","journal-title":"Exp. 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