{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T20:44:53Z","timestamp":1744058693158},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,5,11]],"date-time":"2007-05-11T00:00:00Z","timestamp":1178841600000},"content-version":"vor","delay-in-days":40,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract<\/jats:sc>:\u2002<\/jats:bold>  <jats:bold>Rodent immune\u2010mediated diabetes model studies have advanced understanding of \u03b2 cell\u2013specific T cell responses, and the testing of therapeutic approaches. We have used an inducible diabetes model based on rat insulin promotor (RIP)\u2013driven expression of CD80 (B7\u20101) on pancreatic \u03b2 cells. Using these mice, we have established that immunizing with a single autoantigen can promote progressive islet inflammation and eventually T cell\u2013mediated diabetes. We now describe a potent immunization protocol using peptide\u2010pulsed mature dendritic cells (DCs) to examine peptide epitopes derived from endogenous (preproinsulin) and transgenically expressed \u03b2 cell antigens, namely lymphocytic choriomeningitis virus glycoprotein (LCMV\u2010GP). LCMV\u2010GP epitopes efficiently promote \u03b2 cell destruction, and the autoantigenic peptide concentration used to load the DCs correlates directly with diabetes onset. The system allowed us to assess cytotoxic T cell (CTL) fine specificity by immunizing with DCs presenting altered peptide ligands (APLs) of the dominant LCMV\u2010GP epitope, gp33. Finally, using an adoptive transfer system, we tested alternative <jats:italic>in vitro<\/jats:italic> T cell activation conditions, including APLs and mitogens, for their impact on T cell effector function and diabetes onset. Our studies revealed a marked discrepancy between (inflammatory) effector functions and diabetes progression, thus emphasizing the importance of structural identity between sensitizing and target epitope and the context of initial T cell activation.<\/jats:bold> <\/jats:p>","DOI":"10.1196\/annals.1394.008","type":"journal-article","created":{"date-parts":[[2007,5,15]],"date-time":"2007-05-15T20:19:35Z","timestamp":1179260375000},"page":"132-142","source":"Crossref","is-referenced-by-count":4,"title":["Cytotoxic T Cell\u2013Mediated Diabetes in RIP\u2010CD80 Transgenic Mice"],"prefix":"10.1111","volume":"1103","author":[{"given":"KLAUS","family":"PECHHOLD","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SAGARIKA","family":"CHAKRABARTY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DAVID M.","family":"HARLAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,5,11]]},"reference":[{"key":"e_1_2_4_2_2","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1288.012"},{"key":"e_1_2_4_3_2","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1288.010"},{"key":"e_1_2_4_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/jaut.2000.0380"},{"key":"e_1_2_4_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.8.3137"},{"key":"e_1_2_4_6_2","doi-asserted-by":"publisher","DOI":"10.2337\/diab.44.7.816"},{"key":"e_1_2_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/1074-7613(95)90062-4"},{"key":"e_1_2_4_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.11.5138"},{"key":"e_1_2_4_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.082120099"},{"key":"e_1_2_4_10_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.4.2531"},{"key":"e_1_2_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-8411(02)00105-1"},{"key":"e_1_2_4_12_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.51.11.3237"},{"key":"e_1_2_4_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/12465"},{"key":"e_1_2_4_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1998.tb01202.x"},{"key":"e_1_2_4_15_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.175.2.545"},{"key":"e_1_2_4_16_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.1.86"},{"key":"e_1_2_4_17_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.3.1093"},{"key":"e_1_2_4_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.71.8.5764-5768.1997"},{"key":"e_1_2_4_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002510050595"},{"key":"e_1_2_4_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/icb.1997.16"},{"key":"e_1_2_4_21_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.8.1493"},{"key":"e_1_2_4_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/342559a0"},{"key":"e_1_2_4_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830230315"},{"key":"e_1_2_4_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.69.4.2297-2305.1995"}],"container-title":["Annals of the New York Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1196%2Fannals.1394.008","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1196\/annals.1394.008","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T09:22:04Z","timestamp":1695633724000},"score":1,"resource":{"primary":{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/10.1196\/annals.1394.008"}},"subtitle":["Autoantigen Peptide Sensitivity and Fine Specificity"],"short-title":[],"issued":{"date-parts":[[2007,4]]},"references-count":23,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,4]]}},"alternative-id":["10.1196\/annals.1394.008"],"URL":"https:\/\/doi.org\/10.1196\/annals.1394.008","archive":["Portico"],"relation":{},"ISSN":["0077-8923","1749-6632"],"issn-type":[{"value":"0077-8923","type":"print"},{"value":"1749-6632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,4]]}}}