{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T22:35:20Z","timestamp":1781303720644,"version":"3.54.1"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,8,29]],"date-time":"2007-08-29T00:00:00Z","timestamp":1188345600000},"content-version":"vor","delay-in-days":28,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract<\/jats:sc>:\u2002<\/jats:bold>  <jats:bold>Antibodies to double\u2010stranded desoxyribonucleic acid (dsDNA) and to the polymyositis\/scleroderma (PM\/Scl) complex are regarded as serological markers for systemic lupus erythematosus (SLE) and PM\/Scl overlap syndrome, respectively. In a previous study, serum samples were identified that contained antibodies specific for both dsDNA and PM\/Scl. Fourteen of these sera were available for more detailed investigation including the autoantibody profile as determined by several methods including an addressable laser bead assay, <jats:italic>Crithidia luciliae<\/jats:italic> indirect immunofluorescence test (CLIFT) and a PM1\u2010Alpha ELISA. Moreover, 300 samples from connective tissue disease patients and 30 PM\/Scl positive samples were screened for anti\u2010dsDNA<jats:sup>+<\/jats:sup>\/PM\/Scl<jats:sup>+<\/jats:sup> specimens by CLIFT, dsDNA ELISA, and PM1\u2010Alpha ELISA. We confirmed anti\u2010dsDNA and anti\u2010PM\/Scl reactivity in 2\/7 samples from the previous study. One sample had also anti\u2010chromatin and anti\u2010SS\u2010A reactivity and the second sample was oligoreactive. In addition, 2\/300 (0.7%) unselected samples from connective tissue disease patients were identified with anti\u2010dsDNA and anti\u2010PM\/Scl reactivity. In a panel of PM1\u2010Alpha positive samples (<jats:italic>n<\/jats:italic>= 30) collected regardless of the diagnosis of the patients, no anti\u2010dsDNA reactivity was found. All anti\u2010dsDNA<jats:sup>+<\/jats:sup>\/anti\u2010PM\/Scl<jats:sup>+<\/jats:sup> patients identified fulfilled sufficient criteria to be classified as definite SLE and also had at least one feature of systemic sclerosis (i.e., sclerodactyly and\/or Raynaud's phenomenon). Only 1\/4 patients had clinical evidence of dermatomyositis. The combination of anti\u2010dsDNA<jats:sup>+<\/jats:sup>\/anti\u2010PM\/Scl<jats:sup>+<\/jats:sup> in patients suffering from connective tissue disease is less frequently found than previously described when newer assays are used. Clinically, anti\u2010dsDNA<jats:sup>+<\/jats:sup>\/anti\u2010PM\/Scl<jats:sup>+<\/jats:sup> patients may define a small subgroup of SLE patients with additional features of systemic sclerosis.<\/jats:bold> <\/jats:p>","DOI":"10.1196\/annals.1398.037","type":"journal-article","created":{"date-parts":[[2007,9,4]],"date-time":"2007-09-04T05:08:39Z","timestamp":1188882519000},"page":"311-321","source":"Crossref","is-referenced-by-count":8,"title":["Serological and Clinical Characterization of Anti\u2010dsDNA and Anti\u2010PM\/Scl Double\u2010Positive Patients"],"prefix":"10.1111","volume":"1109","author":[{"given":"M.","family":"MAHLER","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. L.","family":"GREIDINGER","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"SZMYRKA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"KROMMINGA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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