{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T14:07:17Z","timestamp":1745849237101},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,8,29]],"date-time":"2007-08-29T00:00:00Z","timestamp":1188345600000},"content-version":"vor","delay-in-days":28,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract<\/jats:sc>:\u2002<\/jats:bold>  <jats:bold>Patients with celiac disease are sensitive to the gluten fractions of wheat. Symptoms include gastrointestinal problems and a failure to thrive in children, but may range from headaches to general malaise in adults. Thus, it is difficult to diagnose celiac disease by symptoms alone. The standard diagnostic criteria include the presence of the characteristic anti\u2010gliadin or anti\u2010tissue transglutaminase antibodies (anti\u2010tTG) in serum, flattened mucosa on intestinal biopsy, and improved symptoms on a gluten\u2010free diet. Because of the ease of use of the tTG enzyme\u2010linked immunosorbent assay (ELISA) compared to endomysial by indirect immunofluorescence assay, there has been much more screening for celiac disease in recent years. This increased screening showed that celiac disease was more prevalent than previously believed. We compared a new multiplex assay that includes a novel form of deamidated gliadin and recombinant human tTG as the antigens to other assays using standard antigens. In addition, the new assay detects the presence of selective IgA deficiency, which shows a 10\u2010fold increase in prevalence in patients with celiac disease compared to the general population. The combination of sensitivity and specificity of the new multiplex assay was equal or better than those for standard assays. Thus the performance, ease of use, and ability to measure three clinically important parameters in a single test make the new multiplex assay a viable alternative to standard assays in a clinical lab.<\/jats:bold> <\/jats:p>","DOI":"10.1196\/annals.1398.039","type":"journal-article","created":{"date-parts":[[2007,9,4]],"date-time":"2007-09-04T05:08:39Z","timestamp":1188882519000},"page":"330-337","source":"Crossref","is-referenced-by-count":5,"title":["Multiplex Assays to Diagnose Celiac Disease"],"prefix":"10.1111","volume":"1109","author":[{"given":"IVO","family":"LOCHMAN","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"PETER","family":"MARTIS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"RUFUS W.","family":"BURLINGAME","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ALEXANDRA","family":"LOCHMANOV\u00c1","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,8,29]]},"reference":[{"key":"e_1_2_2_2_2","first-page":"1","article-title":"Hepatology and Nutrition","volume":"40","author":"Guideline for the Diagnosis and Treatment of Celiac Disease in Children","year":"2005","journal-title":"JPGN"},{"key":"e_1_2_2_3_2","unstructured":"NIH Consensus Development Conference on Celiac Disease.2004.http:\/\/consensus.nih.gov\/2004\/2004CeliacDisease118html.htm"},{"key":"e_1_2_2_4_2","first-page":"3","article-title":"Celiac disease \u2013 more common than previously assumed AESCU","volume":"2","author":"Schultz M.","year":"2005","journal-title":"Science"},{"key":"e_1_2_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0797-797"},{"key":"e_1_2_2_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.191.4.603"},{"key":"e_1_2_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2006.05.004"},{"key":"e_1_2_2_8_2","doi-asserted-by":"publisher","DOI":"10.2741\/1921"},{"key":"e_1_2_2_9_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M204521200"},{"key":"e_1_2_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0306885101"},{"key":"e_1_2_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/73200"},{"key":"e_1_2_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2004.30296.x"},{"key":"e_1_2_2_13_2","doi-asserted-by":"crossref","first-page":"2023","DOI":"10.1093\/clinchem\/47.11.2023","article-title":"Celiac disease: antibody recognition against native and selectively deamidated gliadin peptides","volume":"47","author":"Aleanzi M.","year":"2001","journal-title":"Clin. Chem."},{"key":"e_1_2_2_14_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.036111"},{"key":"e_1_2_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.13.1.150-151.2006"},{"key":"e_1_2_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2006.02.013"},{"key":"e_1_2_2_17_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.07.054"},{"key":"e_1_2_2_18_2","first-page":"1341","article-title":"Myosin IXB variant increases the risk of celiac disease and points toward a primary intestinal barrier defect","volume":"37","author":"Monsuur A.J.","year":"2005","journal-title":"Genet"},{"key":"e_1_2_2_19_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2005.086637"},{"key":"e_1_2_2_20_2","doi-asserted-by":"publisher","DOI":"10.2741\/1789"},{"key":"e_1_2_2_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-004-0091-7"},{"key":"e_1_2_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2006.03.002"},{"key":"e_1_2_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2004.08.011"},{"key":"e_1_2_2_24_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2003.024836"},{"key":"e_1_2_2_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2004.30296.x"},{"key":"e_1_2_2_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0797-725"},{"key":"e_1_2_2_27_2","first-page":"83","article-title":"Significance of smooth muscle\/anti\u2010actin autoantibodies in celiac disease","volume":"35","author":"Pedreira S.","year":"2005","journal-title":"Acta Gastroenterol. Latinoam."},{"key":"e_1_2_2_28_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.041665"},{"key":"e_1_2_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(92)91819-P"},{"key":"e_1_2_2_30_2","first-page":"1054","article-title":"Evaluation of multiplexed fluorescent microsphere immunoassay for detection of autoantibodies to nuclear antigens","volume":"11","author":"Martins T.B.","year":"2004","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"e_1_2_2_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2000.02018.x"},{"key":"e_1_2_2_32_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118114"},{"key":"e_1_2_2_33_2","doi-asserted-by":"publisher","DOI":"10.1080\/00365520500235334"},{"key":"e_1_2_2_34_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.52.2.218"}],"container-title":["Annals of the New York Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1196%2Fannals.1398.039","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1196\/annals.1398.039","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T10:52:37Z","timestamp":1695639157000},"score":1,"resource":{"primary":{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/10.1196\/annals.1398.039"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,8]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,8]]}},"alternative-id":["10.1196\/annals.1398.039"],"URL":"https:\/\/doi.org\/10.1196\/annals.1398.039","archive":["Portico"],"relation":{},"ISSN":["0077-8923","1749-6632"],"issn-type":[{"value":"0077-8923","type":"print"},{"value":"1749-6632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,8]]}}}