{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:00:41Z","timestamp":1791046841158,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,8,29]],"date-time":"2007-08-29T00:00:00Z","timestamp":1188345600000},"content-version":"vor","delay-in-days":28,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract<\/jats:sc>:\u2002<\/jats:bold>  <jats:bold>\n            <jats:italic>De novo<\/jats:italic> autoimmune hepatitis (AIH) occurs after liver transplantation for nonautoimmune disorders. Autoantibodies so\u2010called atypical anti\u2010liver\/kidney microsome antibodies (LKMA) with an unusual liver\/kidney cytoplasmic staining as judged by indirect immunofluorescence, can be detected in some patients' sera. Few studies investigated their molecular targets, and the aim of this work was to identify the atypical anti\u2010LKMA targets by proteomic tool. This proteomic approach consisted of (<jats:italic>a<\/jats:italic>) two\u2010dimensional gel electrophoresis of cytosolic and microsomal proteins obtained by differential centrifugations of rat liver and rat kidney, followed by (<jats:italic>b<\/jats:italic>) two\u2010dimensional immunoblotting with sera of patients with <jats:italic>de novo<\/jats:italic> AIH (<jats:italic>n<\/jats:italic>= 8, including 2 with anti\u2010LKMA antibodies) and then (<jats:italic>c<\/jats:italic>) identifications of interest spots performed by ion trap mass spectrometry. By this way several proteins at 25 kDa were unambiguously identified: isoforms of carbonic anhydrase III, members of different glutathione S\u2010transferase (GST) families, and subunit \u03b21 of proteasome. This is the first report of proteasome and carbonic anhydrase III as autoantigens in <jats:italic>de novo<\/jats:italic> AIH. These results could lead to a better diagnosis of this disease using identified autoantigens in diagnostic tests, and strengthen proteomic approach as a new way of autoantigens investigation<\/jats:bold>.<\/jats:p>","DOI":"10.1196\/annals.1398.041","type":"journal-article","created":{"date-parts":[[2007,9,4]],"date-time":"2007-09-04T05:08:39Z","timestamp":1188882519000},"page":"345-357","source":"Crossref","is-referenced-by-count":32,"title":["Identification by Proteomic Tool of Atypical Anti\u2010Liver\/Kidney Microsome Autoantibodies Targets in <i>de Novo<\/i> Autoimmune Hepatitis after Liver Transplantation"],"prefix":"10.1111","volume":"1109","author":[{"given":"ST\u00c9PHANIE","family":"HUGUET","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JOELLE","family":"VINH","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"CATHERINE","family":"JOHANET","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DIDIER","family":"SAMUEL","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MICH\u00c8LE","family":"GIGOU","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"OANA","family":"ZAMFIR","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JEAN\u2010CHARLES","family":"DUCLOS\u2010VALL\u00c9E","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ERIC","family":"BALLOT","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,8,29]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)06478-7"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(99)80297-9"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2002.31167"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2003.10.022"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2001.01682.x"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21318"},{"key":"e_1_2_5_8_2","first-page":"99","article-title":"Clinical proteomics: present and future prospects","volume":"27","author":"Verrills N.M.","year":"2006","journal-title":"Clin. 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