{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T09:56:06Z","timestamp":1773309366354,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,12,10]],"date-time":"2007-12-10T00:00:00Z","timestamp":1197244800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2007,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Closed head injury (CHI) often results in profound brain swelling and instant death of the victims due to compression of the vital centers. However, the neurochemical basis of edema formation in CHI is still obscure. Previous studies from our laboratory show that blockade of serotonin synthesis prior to CHI in a rat model attenuates brain edema, indicating a prominent role for serotonin in head injury. Thus, neutralization of endogenous serotonin activity and\/or blocking of its receptors will induce neuroprotection in CHI. Since serotonin has more than 14 receptors and selective serotonin antagonists are still not available, we used serotonin antiserum to neutralize its <jats:italic>in vivo<\/jats:italic> effects before or after CHI in a rat model. CHI was produced by an impact of 0.224 N on the right parietal skull bone under Equithesin anesthesia by dropping a weight of 114.6\u2003g from a height of 20\u2003cm through a guide tube. This concussive brain injury resulted in blood\u2013brain barrier (BBB) disruption, brain edema formation, and volume swelling at 5\u2003h that were most pronounced in the contralateral cerebral hemisphere. The plasma and brain serotonin levels were increased several\u2010fold at this time. Intracerebroventricular administration of serotonin antiserum (1:20, monoclonal) into the left lateral cerebral ventricle (30\u2003\u03bcL in PBS) 30\u2003min before or 30\u2003min (but not 60\u2003min) after CHI significantly attenuated BBB disruption, brain edema formation, volume swelling, and brain pathology. The plasma and brain serotonin levels continued to remain high. These observations are the first to suggest that antiserum to serotonin when administered into the CSF during the early phase of CHI are capable of inducing neuroprotection.<\/jats:bold><\/jats:p>","DOI":"10.1196\/annals.1403.022","type":"journal-article","created":{"date-parts":[[2007,12,12]],"date-time":"2007-12-12T15:57:47Z","timestamp":1197475067000},"page":"295-312","source":"Crossref","is-referenced-by-count":42,"title":["Antibodies to Serotonin Attenuate Closed Head Injury Induced Blood\u2013Brain Barrier Disruption and Brain Pathology"],"prefix":"10.1111","volume":"1122","author":[{"given":"H.S.","family":"SHARMA","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"PATNAIK","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"PATNAIK","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"MOHANTY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"SHARMA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"VANNEMREDDY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,12,10]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/02688690220148824"},{"key":"e_1_2_6_3_2","volume-title":"Traumatic brain injury incidences in children hospitalized or fatal TBIs incidents in seven states","author":"Thurman D.J.","year":"1994"},{"key":"e_1_2_6_4_2","volume-title":"CSN Injury data. Childhood Injury. Cost and Prevention Facts","author":"Miller T.","year":"1994"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/3-211-30714-1_34"},{"key":"e_1_2_6_6_2","first-page":"177","article-title":"Influence of ambient temperature and drug treatments on brain oedema induced by impact injury on skull in rats","volume":"28","author":"Dey P.K.","year":"1984","journal-title":"Indian J. Physiol. Pharmacol."},{"key":"e_1_2_6_7_2","first-page":"554","article-title":"Ambient temperature and development of traumatic brain oedema in anaesthetized animals","volume":"77","author":"Dey P.K.","year":"1983","journal-title":"Indian J. Med. Res."},{"key":"e_1_2_6_8_2","first-page":"91","article-title":"p\u2010Chlorophenylalanine, an inhibitor of serotonin synthesis reduces blood\u2010brain barrier permeability, cerebral blood flow, edema formation and cell injury following trauma to the rat brain","volume":"76","author":"Sharma H.S.","year":"2000","journal-title":"Acta Neurochir Suppl."},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012639011-7\/50016-4"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)91029-G"},{"key":"e_1_2_6_11_2","first-page":"28","volume-title":"Current Drugs, ID Weekly Highlights","author":"Sharma H.S.","year":"2000"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-9489-2_20"},{"key":"e_1_2_6_13_2","first-page":"322","article-title":"Characterization of mianserin neuroprotection in experimental spinal trauma: dose\/route response and late treatment","volume":"269","author":"Salzman S.K.","year":"1994","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410300405"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(92)90013-V"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(91)90063-D"},{"key":"e_1_2_6_17_2","first-page":"175","article-title":"Multiple serotonin receptor subtypes: molecular cloning and functional expression","volume":"8","author":"Teitler M.","year":"1994","journal-title":"Crit. Rev. Neurobiol."},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/3-540-28082-0_3"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2047874"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-409X(97)00096-3"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1636085"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)39332-9"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1058-6687(05)80028-2"},{"key":"e_1_2_6_24_2","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/S0021-9258(17)31252-8","article-title":"A monoclonal antibody against the platelet fibrinogen receptor contains a sequence that mimics a receptor recognition domain in fibrinogen","volume":"264","author":"Taub R.","year":"1989","journal-title":"J. Biol. Chem."},{"key":"e_1_2_6_25_2","first-page":"155","article-title":"Topical application of 5\u2010HT antibodies reduces edema and cell changes following trauma of the rat spinal cord","volume":"70","author":"Sharma H.S.","year":"1997","journal-title":"Acta. Neurochir. Suppl."},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s003290050108"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1602\/neurorx.2.3.410"},{"key":"e_1_2_6_28_2","first-page":"317","volume-title":"Pharmacology of Cerebral Ischemia 1988","author":"Sharma H.S.","year":"1989"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(86)90036-5"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-3452-5_23"},{"key":"e_1_2_6_31_2","first-page":"1","article-title":"Pathophysiology of blood\u2013brain barrier, brain edema and cell injury following hyperthermia. New role of heat shock protein, nitric oxide and carbon monoxide: an experimental study in the rat using light and electron microscopy","volume":"830","author":"Sharma H.S.","year":"1999","journal-title":"Acta Universitatis Upsaliensis"},{"key":"e_1_2_6_32_2","first-page":"1","volume-title":"The Blood","author":"Rapoport S.I.","year":"1976"},{"key":"e_1_2_6_33_2","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1152\/ajplegacy.1949.157.1.122","article-title":"Measurement of experimentally induced brain swelling and shrinkage","volume":"157","author":"Elliott K.A.C.","year":"1949","journal-title":"Am. J. Physiol."},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(06)62010-4"},{"key":"e_1_2_6_35_2","first-page":"1","volume-title":"Current Protocols in Toxicology (Solicited Contribution)","author":"Sharma H.S.","year":"2005"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-0102(88)90051-X"},{"key":"e_1_2_6_37_2","unstructured":"Sharma H.S.1982.Blood\u2013Brain Barrier in Stress Ph.D. Thesis Banaras Hindu University Varanasi India pp.1\u201385."},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-001-0137-z"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-1054-7_8"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(08)62043-9"},{"key":"e_1_2_6_41_2","first-page":"65","article-title":"Role of serotonin and prostaglandins in brain edema induced by heat stress: an experimental study in the young rat","volume":"60","author":"Sharma H.S.","year":"1994","journal-title":"Acta. Neurochir. Suppl. (Wien.)"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(93)90076-R"},{"key":"e_1_2_6_43_2","unstructured":"Ruan J.S.1994.Impact biomechanics of head injury by mathematical modeling.Ph.D. thesis. Wayne State University Detroit MI . pp.1\u2013262."},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1007\/3-211-30714-1_50"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-4328(96)00170-2"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1007\/3-211-30714-1_66"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1007\/3-211-30714-1_62"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-7091-0651-8_85"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-0102(95)01015-7"},{"key":"e_1_2_6_50_2","first-page":"25","volume-title":"Brain Oedema","author":"Mohanty S.","year":"1985"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(08)61803-8"}],"container-title":["Annals of the New York Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1196%2Fannals.1403.022","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1196%2Fannals.1403.022","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1196\/annals.1403.022","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,7]],"date-time":"2023-10-07T10:23:29Z","timestamp":1696674209000},"score":1,"resource":{"primary":{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/10.1196\/annals.1403.022"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,12]]},"references-count":50,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,12]]}},"alternative-id":["10.1196\/annals.1403.022"],"URL":"https:\/\/doi.org\/10.1196\/annals.1403.022","archive":["Portico"],"relation":{},"ISSN":["0077-8923","1749-6632"],"issn-type":[{"value":"0077-8923","type":"print"},{"value":"1749-6632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,12]]}}}