{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T01:12:14Z","timestamp":1722215534399},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,10,10]],"date-time":"2007-10-10T00:00:00Z","timestamp":1191974400000},"content-version":"vor","delay-in-days":39,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract<\/jats:sc>:\u2002<\/jats:bold>  <jats:bold>The coccidioidal T27K vaccine is protective in mice against respiratory challenge with <jats:italic>Coccidioides posadasii<\/jats:italic> (<jats:italic>C. posadasii<\/jats:italic>) arthroconidia. The vaccine is a subcellular multicomponent preparation that has not been fully characterized. To identify potential protective antigens in the heterogeneous mixture, the vaccine has been separated by two\u2010dimensional gel electrophoresis and then analyzed for seroreactive proteins using immunoblot analysis with pooled sera from patients with coccidioidomycosis. Two seroreactive spots of identical apparent molecular weight were identified and sequenced using tandem mass spectrometry. Three peptides were generated, two of which matched a tentative consensus sequence in the TIGR <jats:italic>C<\/jats:italic>. <jats:italic>posadasii<\/jats:italic> 2.0 gene index database that is similar to fungal 1,2\u2010\u03b1\u2010mannosidases. The 5\u2032 and 3\u2032 ends of the mannosidase cDNA were mapped using rapid amplification of cDNA ends (RACE) polymerase chain reaction (PCR), and a full\u2010length cDNA was then obtained using reverse\u2010transcription (RT) PCR. The cDNA was cloned and sequenced and expressed as a recombinant protein. The predicted protein consists of 519 amino acids, has a theoretical molecular weight and pI of 56,918 Da and 4.84, respectively, and is very similar (&gt;60%) to other fungal 1,2\u2010\u03b1\u2010mannosidases. Class I 1,2\u2010\u03b1\u2010mannosidase enzyme activity was also detected in the T27K vaccine using the substrate, Man\u2010\u03b1\u20101,2\u2010Man\u2010\u03b1\u2010OCH<jats:sub>3<\/jats:sub> in a spectrophotometric assay.<\/jats:bold> <\/jats:p>","DOI":"10.1196\/annals.1406.015","type":"journal-article","created":{"date-parts":[[2007,10,17]],"date-time":"2007-10-17T22:34:18Z","timestamp":1192660458000},"page":"164-180","source":"Crossref","is-referenced-by-count":8,"title":["Molecular Cloning and Expression of a cDNA Encoding a <i>Coccidioides posadasii<\/i> 1,2\u2010\u03b1\u2010Mannosidase Identified in the Coccidioidal T27K Vaccine by Immunoproteomic Methods"],"prefix":"10.1111","volume":"1111","author":[{"given":"JENNINE M.","family":"LUNETTA","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KEIRA A.","family":"SIMMONS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"SUZANNE M.","family":"JOHNSON","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DEMOSTHENES","family":"PAPPAGIANIS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,10,10]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/fgbi.2000.1243"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.2164-0947.1960.tb00711.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"crossref","first-page":"132","DOI":"10.4049\/jimmunol.94.1.132","article-title":"Immunization of mice to Coccidioides immitis: dose, regimen and spherulation stage of killed spherule vaccine","volume":"94","author":"Levine H.B.","year":"1965","journal-title":"J. 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Bass Lake CA April 2."},{"key":"e_1_2_6_9_2","unstructured":"Lunetta J. S.Johnson&D.Pappagianis.2006.Identification of a class I 1 2\u2010\u03b1\u2010mannosidase protein in the coccidioidal T27K vaccine using immunoproteomic methods.Presented at the Sixth International Symposium on Coccidioidomycosis.Stanford CA August 23\u201326."},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1080\/15572536.2003.11833250"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac950914h"},{"key":"e_1_2_6_13_2","first-page":"257","volume-title":"Molecular Biology of Pathogenic Fungi","author":"Zimmermann C.R.","year":"1994"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(82)90515-0"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/6.3.265"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(00)00298-5"},{"key":"e_1_2_6_17_2","doi-asserted-by":"crossref","first-page":"1780","DOI":"10.1016\/S0021-9258(19)45543-9","article-title":"Glycosidases of Aspergillus niger. 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