{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T05:29:30Z","timestamp":1785475770277,"version":"3.56.0"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,10,10]],"date-time":"2007-10-10T00:00:00Z","timestamp":1191974400000},"content-version":"vor","delay-in-days":39,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract<\/jats:sc>:\u2002<\/jats:bold>  <jats:bold>The safety, immunogenicity and efficacy of recombinant Ag2\/PRA106 + CSA chimeric fusion protein (CFP) vaccine in ISS\/Montanide adjuvant\u2013administered intramuscular (IM) was assessed in adult female cynomolgus macaques challenged with <jats:italic>Coccidioides posadasii<\/jats:italic>. Animals received three immunizations with either 5 \u03bcg CFP, 50\u2010\u03bcg CFP, or adjuvant alone and were challenged 4 weeks following the final immunization. Although significant antibody response was produced in response to vaccination, there were no discernable adverse effects, suggesting that the vaccine was well tolerated. Upon intratracheal challenge, all animals showed evidence of disease. Two animals that received 5\u2010\u03bcg doses of CFP were euthanatized prior to the study's end because of severe symptoms. Animals vaccinated with 50\u2010\u03bcg doses of CFP showed evidence of enhanced sensitization compared to adjuvant controls and animals vaccinated with 5\u2010\u03bcg doses of CFP. This was based on higher serum anti\u2010CFP titers, enhanced secretion of interferon\u2010gamma (IFN\u2010\u03b3) from stimulated bronchoalveolar lavage mononuclear cells (BALMC), reduced pulmonary radiologic findings following intratracheal challenge, reduced terminal complement fixation titers, and reduced necropsy findings. Overall the vaccine was well tolerated, induced sensitization, and resulted in a protective response when given at the higher 50\u2010\u03bcg dose. Additional experiments may be needed to optimize the vaccination and to confer greater protection against lethal challenge.<\/jats:bold> <\/jats:p>","DOI":"10.1196\/annals.1406.042","type":"journal-article","created":{"date-parts":[[2007,10,17]],"date-time":"2007-10-17T22:34:18Z","timestamp":1192660458000},"page":"290-300","source":"Crossref","is-referenced-by-count":19,"title":["Safety, Antigenicity, and Efficacy of a Recombinant Coccidioidomycosis Vaccine in Cynomolgus Macaques (<i>Macaca fascicularis<\/i>)"],"prefix":"10.1111","volume":"1111","author":[{"given":"SUZANNE M.","family":"JOHNSON","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"NICHOLAS W.","family":"LERCHE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DEMOSTHENES","family":"PAPPAGIANIS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JOANN L.","family":"YEE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JOHN N.","family":"GALGIANI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RICHARD F.","family":"HECTOR","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,10,10]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1206.060028"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(96)00486-6"},{"key":"e_1_2_6_4_2","doi-asserted-by":"crossref","first-page":"2695","DOI":"10.1128\/iai.64.7.2695-2699.1996","article-title":"Molecular cloning and characterization of Coccidioides immitis Antigen 2 cDNA","volume":"64","author":"Zhu Y.","year":"1996","journal-title":"Infect. 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