{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T07:31:22Z","timestamp":1775028682814,"version":"3.50.1"},"reference-count":12,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,10,10]],"date-time":"2007-10-10T00:00:00Z","timestamp":1191974400000},"content-version":"vor","delay-in-days":39,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract<\/jats:sc>:\u2002<\/jats:bold>  <jats:bold>Molecular studies of the genome of the fungus <jats:italic>Coccidioides<\/jats:italic> have demonstrated two nearly identical, but well\u2010identified species, <jats:italic>Coccidioides immitis<\/jats:italic> and <jats:italic>C. posadasii,<\/jats:italic> known as \u201cCalifornia\u201d and \u201cnon\u2010California\u201d species, respectively. The objective of this study was to determine, through molecular methods, whether both species of <jats:italic>Coccidioides<\/jats:italic> are present in Mexican patients with coccidioidomycosis and to estimate, their geographical distribution in Mexico. We analyzed 56 clinical isolates of <jats:italic>Coccidioides<\/jats:italic> spp. from Mexican patients. Molecular identification of each strain was done by means of real time PCR using TaqMan\u00ae probes to amplify single nucleotide polymorphisms (SNPs) in four target sequences, <jats:italic>loci<\/jats:italic>, named proline 157, proline 174, hexokinase 149 and glucose\u2010synthase 192. SNP analysis identified two of the 56 isolates as <jats:italic>Coccidioides immitis<\/jats:italic> and the remaining 54 as <jats:italic>C. posadasii<\/jats:italic>. The dual probe assay that included proline 157, proline 174 and glucose\u2010synthase 192 gave consistent results on SNP differentiation between the two species. In contrast, the template matching hexokinase 149 gave negative results for any species in 34 samples. Our results did not show geographical overlap of the species, and they also confirmed that <jats:italic>C. posadasii<\/jats:italic> is the most frequent species in Mexico. A vast majority of <jats:italic>C. posadasii<\/jats:italic> strains were localized in the north\u2010central region of the country.<\/jats:bold> <\/jats:p>","DOI":"10.1196\/annals.1406.047","type":"journal-article","created":{"date-parts":[[2007,10,17]],"date-time":"2007-10-17T22:34:18Z","timestamp":1192660458000},"page":"326-335","source":"Crossref","is-referenced-by-count":19,"title":["Molecular Identification of <i>Coccidioides<\/i> Isolates from Mexican Patients"],"prefix":"10.1111","volume":"1111","author":[{"given":"LAURA ROC\u00cdO","family":"CASTA\u00d1\u00d3N\u2010OLIVARES","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"DIEGO","family":"G\u00dcERE\u00d1A\u2010ELIZALDE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MARISELA ROC\u00cdO","family":"GONZ\u00c1LEZ\u2010MART\u00cdNEZ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ALEXEI FEDOROVICH","family":"LICEA\u2010NAVARRO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GLORIA MAR\u00cdA","family":"GONZ\u00c1LEZ\u2010GONZ\u00c1LEZ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ARTURO","family":"AROCH\u2010CALDER\u00d3N","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,10,10]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"crossref","first-page":"3040","DOI":"10.1128\/jcm.32.12.3040-3042.1994","article-title":"Characterization of Coccidioides immitis isolates by restriction fragment length polymorphisms","volume":"32","author":"Zimmermann C.R.","year":"1994","journal-title":"J. Clin. Microbiol."},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.10.5478"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-294X.1999.00655_5.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.071406098"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/15572536.2003.11833250"},{"key":"e_1_2_6_7_2","unstructured":"Barker B. S.Kroken&M.Orbach.2006.Microsatellite analysis of environmental isolates of the human pathogenic fungusCoccidioidesvalidates the genetic and geographical isolation among populations and species. Presented at the Sixth International Symposium on Coccidioidomycosis. Stanford CA August23\u201326."},{"key":"e_1_2_6_8_2","first-page":"145","article-title":"Coccidioidomicosis y su escaso conocimiento en nuestro pa\u00eds","volume":"47","author":"Casta\u00f1\u00f3n Olivares L.R.","year":"2004","journal-title":"Rev. Fac. Med. U. N. A. M."},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.3.1247-1249.2003"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.778-783.2004"},{"key":"e_1_2_6_11_2","volume-title":"U.S. Department of Health and Human Services Centers for Disease Control and Prevention and National Institutes of Health","author":"Biosafety in Microbiological and Biomedical Laboratories","year":"1999"},{"key":"e_1_2_6_12_2","volume-title":"Coccidioidomycosis","author":"Fiese M.J.","year":"1958"},{"key":"e_1_2_6_13_2","volume-title":"Tomo II. Secci\u00f3n IV.4.2 Vientos dominantes durante el a\u00f1o","author":"Atlas Nacional de M\u00e9xico","year":"1989"}],"container-title":["Annals of the New York Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1196%2Fannals.1406.047","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1196\/annals.1406.047","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T14:00:36Z","timestamp":1695650436000},"score":1,"resource":{"primary":{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/10.1196\/annals.1406.047"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,9]]},"references-count":12,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["10.1196\/annals.1406.047"],"URL":"https:\/\/doi.org\/10.1196\/annals.1406.047","archive":["Portico"],"relation":{},"ISSN":["0077-8923","1749-6632"],"issn-type":[{"value":"0077-8923","type":"print"},{"value":"1749-6632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,9]]}}}