{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T00:10:37Z","timestamp":1695687037733},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,10,10]],"date-time":"2007-10-10T00:00:00Z","timestamp":1191974400000},"content-version":"vor","delay-in-days":39,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract<\/jats:sc>:\u2002<\/jats:bold>  <jats:bold>Thymosin fraction\u20105 (TF5) is a protein preparation of the bovine thymus. TF5 stimulates many assays of T cell\u2014mediated immunity. We found that TF5 substantially suppressed proliferation of the rat C6 glioma and MMQ pituitary adenoma cell lines. Our current research using the promyelocytic cell line HL\u201060 suggests that TF5 also prevents proliferation of human myeloid leukemia cells. Our objective is the purification and chemical characterization of TF5 peptide components responsible for inhibition of HL\u201060 proliferative capacity. Using the inhibition of HL\u201060 cell proliferation, we have chemically characterized TF5 using fast protein liquid chromatography (FPLC), reversed\u2010phase high\u2010performance liquid chromatography (RP\u2010HPLC), and high\u2010performance capillary electrophoresis (HPCE). Vital dye\u2010exclusion, oxidative metabolism of chromogenic dyes, and clonogenic growth profiles were used to determine rates of HL\u201060 proliferation. Our results identified an \u223c6000 Da component of TF5 capable of inducing HL\u201060 growth arrest. Synchronized HL\u201060 cells exposed to TF5 and its various constituents were subjected to cytometric analysis by flow cytometry. TF5\u2010treated HL\u201060 cells had an increased subdiploid faction (i.e., sub\u2010G1) compared to control cells. TF5 also increased Annexin V staining in randomly cycling HL\u201060 cells. Thus, a TF5 subfraction possesses growth\u2010suppressive activity for human myeloid neoplasms. Our results indicate that this effect is characterized by at least one hallmark of apoptosis. Future clinical management strategies for certain leukemias may involve the use of thymic peptides.<\/jats:bold> <\/jats:p>","DOI":"10.1196\/annals.1415.022","type":"journal-article","created":{"date-parts":[[2007,10,18]],"date-time":"2007-10-18T19:57:59Z","timestamp":1192737479000},"page":"305-316","source":"Crossref","is-referenced-by-count":2,"title":["Thymosin Fraction\u20105 Possesses Antiproliferative Properties in HL\u201060 Human Promyelocytic Leukemia Cells"],"prefix":"10.1111","volume":"1112","author":[{"given":"BRYAN L.","family":"SPANGELO","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JOSEPH D.","family":"ROACH","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"FREIDUN","family":"HADI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ALI A.","family":"DAMAVANDY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"JORDAN","family":"PLIESKATT","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MAHNAZ","family":"BADAMCHIAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,10,10]]},"reference":[{"key":"e_1_2_7_2_2","first-page":"191","article-title":"Thymosins and other hormones of the thymus gland","volume":"58","author":"Schulof R.S.","year":"1981","journal-title":"Prog. 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