{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T19:38:37Z","timestamp":1791488317071,"version":"4.3.4"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2007,8,29]],"date-time":"2007-08-29T00:00:00Z","timestamp":1188345600000},"content-version":"vor","delay-in-days":89,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:p><jats:bold><jats:sc>Abstract<\/jats:sc>:\u2002<\/jats:bold>  <jats:bold>The aim was to explore the role of prolactin (PRL) in the lymphocyte activation process in systemic lupus erythematosus (SLE) patients in an <jats:italic>in vitro<\/jats:italic> model. Peripheral blood mononuclear cells (PBMCs) were isolated from SLE patients and healthy individuals. The mRNA for PRL and its receptor obtained by standard techniques, with an appropriate primer, were subjected to polymerase chain reaction (PCR) and visualized. The PBMCs were cultured with (<jats:italic>a<\/jats:italic>) medium alone as a negative control, (<jats:italic>b<\/jats:italic>) unspecific mitogen as a positive control, (<jats:italic>c<\/jats:italic>) PRL alone, (<jats:italic>d<\/jats:italic>) mitogen plus PRL, (<jats:italic>e<\/jats:italic>) mitogen plus antibody anti\u2010PRL, and (<jats:italic>f<\/jats:italic>) mitogen plus a nonrelated antibody. Then CD69 and CD154 were determined by flow cytometry analysis. Twelve inactive and 15 active SLE patients were studied. Twenty\u2010five percent of the active patients displayed hyperprolactinemia. Under basal conditions CD69 expression was associated with disease activity. The PBMCs activated <jats:italic>in vitro<\/jats:italic> were capable of producing and secreting PRL, measured by mRNA and Nb2 assay. In a similar way, the mRNA for the PRL receptor was visualized. Cells from SLE patients cultivated with PRL alone did not display increased CD69 and CD154 expression. The addition of PRL to the unspecific stimulated culture does not have an additive effect. In contrast, the addition of antibodies against PRL in order to block the autocrine PRL resulted in a striking reduction of CD69 and CD154 expression.<\/jats:bold> <\/jats:p>","DOI":"10.1196\/annals.1422.018","type":"journal-article","created":{"date-parts":[[2007,10,16]],"date-time":"2007-10-16T22:43:07Z","timestamp":1192574587000},"page":"157-165","source":"Crossref","is-referenced-by-count":18,"title":["Effect of Prolactin on Lymphocyte Activation from Systemic Lupus Erythematosus Patients"],"prefix":"10.1111","volume":"1108","author":[{"given":"KARINA","family":"CHAVEZ\u2010RUEDA","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"VICTORIA MA.","family":"LEGORRETA\u2010HAQUET","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HERNANDO","family":"CERVERA\u2010CASTILLO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LOURDES","family":"S\u00c1NCHEZ","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LUIS JAVIER","family":"JARA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"EDGAR","family":"ZENTENO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LUIS","family":"CHAVEZ\u2010SANCHEZ","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FRANCISCO","family":"BLANCO\u2010FAVELA","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,8,29]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780251101"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780350606"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1991.tb05775.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/096120339200100609"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/smim.1996.0022"},{"key":"e_1_2_6_7_2","doi-asserted-by":"crossref","first-page":"5124","DOI":"10.4049\/jimmunol.155.11.5124","article-title":"B7\/CD28 dependent and independent induction of CD40 ligand expression","volume":"155","author":"Ding L.","year":"1995","journal-title":"J. Immunol."},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1996.tb00921.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(94)90193-7"},{"key":"e_1_2_6_10_2","first-page":"331","article-title":"CD69 expression on neutrophils from patients with rheumatoid arthritis","volume":"22","author":"Atzeni F.","year":"2004","journal-title":"Clin. Exp. Rheumatol"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1006\/jaut.1998.0228"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3083.1998.00366.x"},{"key":"e_1_2_6_13_2","first-page":"807","article-title":"Hyperprolactinaemia in patients with systemic lupus erythematosus","volume":"20","author":"Moszkorzova L.","year":"2002","journal-title":"Clin. Exp. Rheumatol."},{"key":"e_1_2_6_14_2","first-page":"55","article-title":"Association between prolactin and disease activity in SLE. Influence of statistical power","volume":"26","author":"Blanco\u2010Favela F.","journal-title":"J. Rheumatol."},{"key":"e_1_2_6_15_2","first-page":"268","article-title":"Correlation study between prolactin and androgens in male patients with systemic lupus erythematosus","volume":"14","author":"Lavalle C.","year":"1987","journal-title":"J. Rheumatol."},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000441-199204000-00003"},{"key":"e_1_2_6_17_2","first-page":"629","article-title":"No correlation between prolactin levels and clinical activity in patients with systemic lupus erythematosus","volume":"23","author":"Buskila D.","year":"1996","journal-title":"J. Rheumatol."},{"key":"e_1_2_6_18_2","first-page":"2064","article-title":"Prolactin in systemic lupus erythematosus","volume":"21","author":"Pauzner R.","year":"1994","journal-title":"J. Rheumatol."},{"key":"e_1_2_6_19_2","first-page":"476","article-title":"Elevated serum prolactin or elevated prolactin\/cortisol ratio are associated with autoimmune processes in systemic lupus erythematosus and other connective tissue diseases","volume":"23","author":"Neidhart M.","year":"1996","journal-title":"J. Rheumatol."},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.3109\/03009749609069215"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1006\/jaut.1995.0033"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/35.2.133"},{"key":"e_1_2_6_23_2","first-page":"1569","article-title":"Prolactin levels in men with SLE and RA","volume":"17","author":"Folomeev M.","year":"1990","journal-title":"J. Rheumatol."},{"key":"e_1_2_6_24_2","doi-asserted-by":"crossref","first-page":"780","DOI":"10.4049\/jimmunol.147.11.3780","article-title":"Prolactin influences autoimmune disease activity in the female B\/W mouse","volume":"147","author":"McMurray R.","year":"1991","journal-title":"J. Immunol."},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1006\/clin.1994.1095"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3122324"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1570187"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(84)90762-9"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.16.6460"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.3.10.2787766"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0889-1591(92)90039-Q"},{"key":"e_1_2_6_32_2","first-page":"1546","article-title":"Anti\u2010prolactin antibodies in systemic lupus erythematosus patients; frequency and correlation with prolactinemia and disease activity","volume":"7","author":"Lea\u00f1os\u2010Miranda A.","journal-title":"2001"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1177\/096120330101001107"},{"key":"e_1_2_6_34_2","first-page":"506","article-title":"Regulation of humoral immunity in rat by pituitary hormone","volume":"98","author":"Berczi I.","year":"1981","journal-title":"Acta Endocrinol."},{"key":"e_1_2_6_35_2","first-page":"1151","article-title":"Effect of prolactin on HLA\u2010DR and CD40 expressions by human thyrocytes","volume":"114","author":"Li J.","year":"2001","journal-title":"Chin. Med. J."},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.23.6926"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2005.03.013"}],"container-title":["Annals of the New York Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1196%2Fannals.1422.018","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1196\/annals.1422.018","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T03:06:13Z","timestamp":1695697573000},"score":1,"resource":{"primary":{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/10.1196\/annals.1422.018"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,6]]},"references-count":36,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,6]]}},"alternative-id":["10.1196\/annals.1422.018"],"URL":"https:\/\/doi.org\/10.1196\/annals.1422.018","archive":["Portico"],"relation":{},"ISSN":["0077-8923","1749-6632"],"issn-type":[{"value":"0077-8923","type":"print"},{"value":"1749-6632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,6]]}}}