{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T22:42:00Z","timestamp":1781736120044,"version":"3.54.5"},"reference-count":6,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T00:00:00Z","timestamp":1229299200000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:p>Molecular detection of <jats:italic>Babesia bigemina<\/jats:italic> involves a nested PCR protocol and reverse line blot hybridization (RLBH) assay based on the 18S gene. In this study, we report the development of molecular tools for improving <jats:italic>B. bigemina<\/jats:italic> detection in bovine blood\u2014a one\u2010step PCR assay based on the amplification of <jats:italic>rap\u20101a<\/jats:italic> paralogous and a new RLBH <jats:italic>Babesia<\/jats:italic> spp. 18S probe. The one\u2010step PCR assay is highly specific, with an estimated analytical sensitivity corresponding to 0.00002% parasitemia. The RLBH assay, with a new <jats:italic>B. bigemina<\/jats:italic> probe, allows the detection of all tested <jats:italic>B. bigemina<\/jats:italic> isolates showing no cross\u2010hybridization with <jats:italic>B. bovis<\/jats:italic> 18S gene. By developing this highly specific and sensitive one\u2010step PCR and upgrading the RLBH assay for <jats:italic>B. bigemina<\/jats:italic>, we have improved molecular assays which, together with serologic methods, provide valuable tools for epidemiologic studies of bovine babesiosis.<\/jats:p>","DOI":"10.1196\/annals.1428.038","type":"journal-article","created":{"date-parts":[[2009,8,11]],"date-time":"2009-08-11T16:35:06Z","timestamp":1250008506000},"page":"155-157","source":"Crossref","is-referenced-by-count":13,"title":["Improved Molecular Tools for Detection of <i>Babesia bigemina<\/i>"],"prefix":"10.1196","volume":"1149","author":[{"given":"Romina","family":"Petrigh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Ruybal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Neumann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosalia","family":"Moretta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvina","family":"Wilkowsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graciela","family":"Draghi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Echaide","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana Torioni","family":"De Echaide","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisa","family":"Farber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,12,15]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(01)00464-2"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.30.10.2576-2582.1992"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-003-0980-9"},{"key":"e_1_2_6_5_2","first-page":"1","article-title":"Rapid, efficient DNA extraction for PCR from cells or blood","volume":"2","author":"Higuchi R.","year":"1989","journal-title":"Amplifications"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.6.1782-1789.1999"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(02)00311-0"}],"container-title":["Annals of the New York Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1196%2Fannals.1428.038","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1196\/annals.1428.038","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T23:09:11Z","timestamp":1695683351000},"score":1,"resource":{"primary":{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/10.1196\/annals.1428.038"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,12]]},"references-count":6,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,12]]}},"alternative-id":["10.1196\/annals.1428.038"],"URL":"https:\/\/doi.org\/10.1196\/annals.1428.038","archive":["Portico"],"relation":{},"ISSN":["0077-8923","1749-6632"],"issn-type":[{"value":"0077-8923","type":"print"},{"value":"1749-6632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,12]]}}}