{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:21:13Z","timestamp":1791354073838,"version":"4.3.1"},"reference-count":10,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,12,15]],"date-time":"2008-12-15T00:00:00Z","timestamp":1229299200000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:p>Heat shock protein 20 (HSP\u201020) and rhoptry\u2010associated protein 1a (RAP\u20101a) are two proteins considered as candidates to be included in vaccines or diagnostics methods for the control of bovine babesiosis. It has been hypothesized that both genes have a basic function in the cellular physiology of erythrocyte\u2010infecting stages; it is not known if they have a functional role in tick stages. The objective of this work was to analyze whether <jats:italic>hsp\u201020<\/jats:italic> and <jats:italic>rap\u20101a<\/jats:italic> are expressed in sexual stages and kinetes of <jats:italic>Babesia bigemina<\/jats:italic>. Purified RNA from sexual stages and kinetes was analyzed by reverse transcriptase (RT)\u2010PCR with specific primers for <jats:italic>hsp\u201020<\/jats:italic> or <jats:italic>rap\u20101a.<\/jats:italic> Expression analysis was carried out using an indirect immunofluorescence test with specific antibodies against HSP\u201020 and RAP\u20101a. Results obtained by RT\u2010PCR showed amplicons for <jats:italic>hsp\u201020<\/jats:italic> and <jats:italic>rap\u20101a<\/jats:italic> in sexual stages and kinetes. Positive signals were also detected when sexual stages and kinetes were analyzed with specific antibodies for HSP\u201020 and RAP\u20101a. The results obtained here confirm the hypothesis that the genes <jats:italic>hsp\u201020<\/jats:italic> and <jats:italic>rap\u20101a<\/jats:italic> from <jats:italic>B. bigemina<\/jats:italic> are expressed in sexual stages and kinetes and stress the importance of these proteins in the cellular physiology of tick stages.<\/jats:p>","DOI":"10.1196\/annals.1428.073","type":"journal-article","created":{"date-parts":[[2009,8,11]],"date-time":"2009-08-11T16:35:06Z","timestamp":1250008506000},"page":"136-140","source":"Crossref","is-referenced-by-count":11,"title":["Expression Analysis of Heat Shock Protein 20 and Rhoptry\u2010associated Protein 1a in Sexual Stages and Kinetes of <i>Babesia bigemina<\/i>"],"prefix":"10.1196","volume":"1149","author":[{"given":"Rodrigo","family":"Vichido","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfonso","family":"Falcon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan A.","family":"Ramos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Alvarez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio V.","family":"Figueroa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junzo","family":"Norimine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendy C.","family":"Brown","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis A.","family":"Castro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Mosqueda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,12,15]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1071\/AR9640802"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(01)00375-9"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2006.00849.x"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.2.1096-1106.2003"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1307.034"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(02)00311-0"},{"key":"e_1_2_7_8_2","doi-asserted-by":"crossref","first-page":"997","DOI":"10.1128\/jcm.29.5.997-1003.1991","article-title":"In vitro inhibition of multiplication of Babesia bigemina by using monoclonal antibodies","volume":"29","author":"Figueroa J.V.","year":"1991","journal-title":"J. Clin. Microbiol."},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2004.07.003"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.3.1599-1603.2002"},{"key":"e_1_2_7_11_2","doi-asserted-by":"crossref","first-page":"2298","DOI":"10.4049\/jimmunol.138.7.2298","article-title":"Antibodies define multiple proteins with epitopes exposed on the surface of live Babesia bigemina merozoites","volume":"138","author":"McElwain T.F.","year":"1987","journal-title":"J. Immunol."}],"container-title":["Annals of the New York Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1196%2Fannals.1428.073","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/pdf\/10.1196\/annals.1428.073","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T23:10:41Z","timestamp":1695683441000},"score":1,"resource":{"primary":{"URL":"https:\/\/nyaspubs.onlinelibrary.wiley.com\/doi\/10.1196\/annals.1428.073"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,12]]},"references-count":10,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,12]]}},"alternative-id":["10.1196\/annals.1428.073"],"URL":"https:\/\/doi.org\/10.1196\/annals.1428.073","archive":["Portico"],"relation":{},"ISSN":["0077-8923","1749-6632"],"issn-type":[{"value":"0077-8923","type":"print"},{"value":"1749-6632","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,12]]}}}