{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:33:27Z","timestamp":1791275607919,"version":"4.1.0"},"reference-count":12,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,9,16]],"date-time":"2008-09-16T00:00:00Z","timestamp":1221523200000},"content-version":"vor","delay-in-days":46,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Annals of the New York Academy of Sciences"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:p>The DNase activity and circulating DNA (cirDNA) concentration in blood plasma of healthy donors, patients with chronic prostatitis, and patients with prostate tumors were analyzed. The concentration of the cirDNA from plasma was determined by PicoGreen fluorescent assay. DNase activity in blood was measured using the immunoassay based on the cleavage of a hapten\u2010labeled 974\u2010bp DNA substrate. The mean cirDNA concentration in the plasma of healthy donors was low (21 \u00b1 4 ng\/mL total blood) and was accompanied by high DNase activity (0.17 \u00b1 0.04 U\/mL blood). The mean cirDNA concentration was 90 ng\/mL blood (10\u2013234 ng\/mL) in the patients with nonmalignant prostate tumors and 115 ng\/mL blood (13\u2013339 ng\/mL) in those with prostate cancer. The mean DNase activity in blood plasma of the patients with prostate tumors was 0.06 U\/mL blood (0\u20130.12 U\/mL). The results obtained demonstrate that increased concentrations of cirDNA in blood of the patients with prostate tumors is accompanied by a decreased DNase activity, confirming our previous data that a low DNase activity in blood plasma of cancer patients is one reason for a high cirDNA concentration.<\/jats:p>","DOI":"10.1196\/annals.1448.016","type":"journal-article","created":{"date-parts":[[2009,8,11]],"date-time":"2009-08-11T11:29:58Z","timestamp":1249990198000},"page":"218-221","source":"Crossref","is-referenced-by-count":91,"title":["Deoxyribonuclease Activity and Circulating DNA Concentration in Blood Plasma of Patients with Prostate Tumors"],"prefix":"10.1196","volume":"1137","author":[{"given":"Anna V.","family":"Cherepanova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana N.","family":"Tamkovich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga E.","family":"Bryzgunova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentin V.","family":"Vlassov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pavel P.","family":"Laktionov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,9,16]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2001.41020276.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1997.3571249.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2006.10.001"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1134\/S1990750807040038"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19880501)61:9<1838::AID-CNCR2820610920>3.0.CO;2-R"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1368.026"},{"key":"e_1_2_6_8_2","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1007\/BF02773731","article-title":"Clinical investigation of serum deoxyribonuclease. 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