{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T03:14:26Z","timestamp":1771298066395,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2008,6,28]],"date-time":"2008-06-28T00:00:00Z","timestamp":1214611200000},"content-version":"vor","delay-in-days":1366,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Academic Emergency Medicine"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p> <jats:bold>Objectives:<\/jats:bold> Brain edema occurs following clinical as well as experimental cardiac arrest (CA) and predicts a poor neurologic outcome. The objective of this study was to determine the expression of cerebral cortex aquaporin (AQP)\u20104, a member of a family of membrane water\u2010channel proteins, in brain edema formation following normothermic or hypothermic CA. <jats:bold>Methods:<\/jats:bold> Twenty\u2010four rats were subjected to time\u2010matched normothermic (N\u2010Sham, 37.5\u00b0C \u00b1 0.5\u00b0C, <jats:italic>n<\/jats:italic>= 6) or hypothermic (H\u2010Sham, 34\u00b0C \u00b1 0.5\u00b0C, <jats:italic>n<\/jats:italic>= 6) sham experiments and normothermic (N\u2010CA, <jats:italic>n<\/jats:italic>= 6) or hypothermic (H\u2010CA, <jats:italic>n<\/jats:italic>= 6) CA induced by asphyxiation for 8 minutes. Hypothermia was induced before CA. The animals were resuscitated with cardiopulmonary resuscitation, ventilation, and epinephrine administration. Brain edema was determined by brain wet\u2010to\u2010dry weight ratio at one hour of resuscitation. AQP4 immunoactivity in the cerebral cortex was determined using immunohistochemical staining and was semiquantified as an intensity of staining with an automated cell imaging system. <jats:bold>Results:<\/jats:bold> Mild hypothermia in the sham experiments did not alter cerebral cortex AQP4 immunoactivity (mean \u00b1 SD) (55.0 \u00b1 3.7 in H\u2010Sham vs. 53.3 \u00b1 1.7 in N\u2010Sham, p &gt; 0.05). N\u2010CA resulted in a significant increase in AQP4 immunoactivity (61.8 \u00b1 4.5) compared with N\u2010Sham (p = 0.01) and H\u2010Sham (p = 0.03). H\u2010CA attenuated AQP4 compared with N\u2010CA (53.4 \u00b1 1.3, p = 0.01). Brain wet\u2010to\u2010dry weight ratios were 4.41 \u00b1 0.07 in N\u2010Sham, 4.40 \u00b1 0.08 in H\u2010Sham (p &gt; 0.05 vs. N\u2010Sham), 4.55 \u00b1 0.04 in N\u2010CA (p = 0.004 vs. N\u2010Sham; p = 0.005 vs. H\u2010Sham), and 4.43 \u00b1 0.09 in H\u2010CA (p = 0.02 vs. N\u2010CA; p &gt; 0.05 vs. N\u2010Sham and H\u2010Sham). <jats:bold>Conclusions<\/jats:bold> Cerebral cortical AQP4 expression is up\u2010regulated after normothermic CA, which is attenuated by hypothermia induced before CA.<\/jats:p>","DOI":"10.1197\/j.aem.2004.05.026","type":"journal-article","created":{"date-parts":[[2004,10,13]],"date-time":"2004-10-13T02:32:40Z","timestamp":1097634760000},"page":"1001-1007","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Cerebral Cortical Aquaporin\u20104 Expression in Brain Edema following Cardiac Arrest in Rats"],"prefix":"10.1111","volume":"11","author":[{"given":"Feng","family":"Xiao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas C.","family":"Arnold","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Brown","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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