{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T15:53:38Z","timestamp":1792079618337,"version":"4.4.0"},"reference-count":46,"publisher":"American Society of Clinical Oncology (ASCO)","issue":"3","content-domain":{"domain":["ascopubs.org"],"crossmark-restriction":true},"short-container-title":["JCO Glob Oncol"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:sec>\n                    <jats:title>PURPOSE<\/jats:title>\n                    <jats:p>Sarcomas exhibit clinicopathologic heterogeneity, leading to diagnostic and therapeutic uncertainty. This study assesses the clinical value of integrating targeted RNA-based next-generation sequencing (NGS) into a multidisciplinary sarcoma workflow to improve diagnosis, enable comprehensive molecular characterization, and inform personalized treatment strategies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>In this prospective single-center study, 68 patients with suspected or confirmed soft tissue sarcoma underwent RNA-based NGS using a 138-gene fusion panel, integrated within a multidisciplinary sarcoma framework. Genomic findings integrated with histopathology and immunohistochemistry for diagnostic reclassification and treatment changes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Of 70 patients advised RNA-based NGS, 68 (97.1%) were evaluable (median age, 38.5 years). Soft tissue sarcomas predominated (77.1%), with 67.1% presenting with advanced disease. Initial histology included 17 round cell sarcomas (RC), 19 undifferentiated sarcomas (US), and 34 other subtypes. Among evaluable RC cases (n = 16), NGS identified diagnostic fusions in 87.5%, enabling reclassification in 68.7% and treatment change in 18.7%. In US, fusions were detected in 21.1%, all reclassified, with treatment altered in 75%. In other subtypes, fusions were found in 48.4%, enabling reclassification in 39.3% and treatment impact in 21.2%. Nondiagnostic mutations (eg,\n                      <jats:italic toggle=\"yes\">TP53<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">SLX4<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">NF1<\/jats:italic>\n                      ) were observed in 47.4% of US and 18.2% of other subtypes. Potentially actionable variants (\n                      <jats:italic toggle=\"yes\">KIT<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">PIK3CA<\/jats:italic>\n                      ) were seen in 5.8%. Overall, RNA-NGS was clinically informative in 50%, refined diagnosis in 41.1%, and influenced treatment in 26.4% of patients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>To our knowledge, this first-of-its-kind study highlights the clinical utility of integrating targeted RNA-based NGS into multidisciplinary sarcoma care, enabling diagnostic reclassification in 41.1% of cases most notably in round cell sarcomas with atypical morphology. Both canonical and rare fusions informed individualized therapy, reinforcing RNA-NGS as a pivotal tool for diagnostic refinement and precision oncology in sarcoma management.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1200\/go-25-00440","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T19:59:09Z","timestamp":1773259149000},"update-policy":"https:\/\/doi.org\/10.1200\/crossmark","source":"Crossref","is-referenced-by-count":5,"title":["Clinical Impact of Integrating RNA-Based Next-Generation Sequencing Into the Diagnostic Evaluation of Soft Tissue Sarcomas: Insights From a Single-Center Multidisciplinary Workflow"],"prefix":"10.1200","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6126-4005","authenticated-orcid":true,"given":"Nihanthy D.","family":"Sreenath","sequence":"first","affiliation":[{"name":"Saroj Gupta Cancer Centre and Research Institute, Kolkata, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-2501-6600","authenticated-orcid":true,"given":"Kinjal","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4792-3083","authenticated-orcid":true,"given":"Sameer","family":"Rastogi","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niranjan","family":"Biswal","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6448-9761","authenticated-orcid":true,"given":"Akash","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4805-3342","authenticated-orcid":true,"given":"Adarsh","family":"Barwad","sequence":"additional","affiliation":[{"name":"Department of Pathology, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7978-428X","authenticated-orcid":true,"given":"Asit Ranjan","family":"Mridha","sequence":"additional","affiliation":[{"name":"Department of Pathology, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivanand","family":"Gamanagatti","sequence":"additional","affiliation":[{"name":"Department of Radio-diagnosis, Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8787-4285","authenticated-orcid":true,"given":"Shamim Ahmed","family":"Shamim","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1666-3354","authenticated-orcid":true,"given":"Yamini","family":"Dharmashaktu","sequence":"additional","affiliation":[{"name":"Department of Nuclear Medicine, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charu","family":"Bahl","sequence":"additional","affiliation":[{"name":"MedGenome Labs Ltd, Bengaluru, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aparna","family":"Natrajan","sequence":"additional","affiliation":[{"name":"MedGenome Labs Ltd, Bengaluru, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8265-9674","authenticated-orcid":true,"given":"Ekta","family":"Dhamija","sequence":"additional","affiliation":[{"name":"Department of Radio-diagnosis, Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3509-4794","authenticated-orcid":true,"given":"Bharat","family":"Rekhi","sequence":"additional","affiliation":[{"name":"Department of Pathology, Tata Memorial Hospital (TMH), Homi Bhabha National Institute (HBNI) University, Parel, Mumbai, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6392-6578","authenticated-orcid":true,"given":"Stanzin","family":"Spalkit","sequence":"additional","affiliation":[{"name":"Department of Radio-diagnosis, Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"233","reference":[{"key":"e_1_3_5_2_2","volume-title":"StatPearls","author":"Popovich JR","year":"2025","unstructured":"Popovich JR, Kashyap S, Gasalberti DP, et al: Sarcoma, in StatPearls. Treasure Island, FL, StatPearls Publishing, 2025"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-024-01650-6"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02306088"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.32074\/1591-951X-213"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/cup.13870"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2025.1525722"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22050"},{"key":"e_1_3_5_9_2","unstructured":"Kao E Belzarena C: Spindle cell\/sclerosing rhabdomyosarcoma. PathologyOutlines.com"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22978"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2024.01.007"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2016-0417-RA"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24065934"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(15)00583-5"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-30496-0"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2022.102455"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2025.43.16_suppl.11541"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.1177\/10668969241246462"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.3332\/ecancer.2022.1459"},{"key":"e_1_3_5_20_2","unstructured":"Reference deleted"},{"key":"e_1_3_5_21_2","first-page":"e98552","article-title":"Clinico-pathological and clinical outcomes of soft tissue sarcoma with FUS-TFCP2 or EWSR1-TFCP2 fusions: A case series from North India","volume":"17","author":"Agarwal K","year":"2025","unstructured":"Agarwal K, Rastogi S, Pasricha S, et al: Clinico-pathological and clinical outcomes of soft tissue sarcoma with FUS-TFCP2 or EWSR1-TFCP2 fusions: A case series from North India. Cureus. 17:e98552, 2025","journal-title":"Cureus"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1177\/10668969241301060"},{"key":"e_1_3_5_23_2","unstructured":"Reference deleted"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-019-0323-8"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.hpr.2021.300552"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.esmoop.2022.100522"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15245750"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15174378"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2022.40.16_suppl.e23542"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.23226"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers14122980"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-024-03995-2"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.23176"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.4103\/ijpm.ijpm_275_24"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22132"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001147"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22839"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2017.162"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.semdp.2023.04.014"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.3332\/ecancer.2025.1907"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15143584"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.5306\/wjco.v10.i5.213"},{"key":"e_1_3_5_43_2","unstructured":"Carmagnani PR Groisberg R Roszik J et al: Precision oncology in sarcomas: Divide and conquer. JCO Precis Oncol 10.1200\/PO.18.00247"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.25259\/IJMIO_6_2021"},{"key":"e_1_3_5_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20603-4"},{"key":"e_1_3_5_46_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-024-13159-9"},{"key":"e_1_3_5_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-01649-w"}],"container-title":["JCO Global Oncology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/ascopubs.org\/doi\/pdfdirect\/10.1200\/GO-25-00440","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T19:59:17Z","timestamp":1773259157000},"score":1,"resource":{"primary":{"URL":"https:\/\/ascopubs.org\/doi\/10.1200\/GO-25-00440"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3]]},"references-count":46,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1200\/GO-25-00440"],"URL":"https:\/\/doi.org\/10.1200\/go-25-00440","relation":{},"ISSN":["2687-8941"],"issn-type":[{"value":"2687-8941","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3]]},"assertion":[{"value":"2025-08-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-08","order":1,"name":"revised","label":"Revised","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e2500440"}}